Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity

Hayati Journal of Biosciences

TOPIK HIDAYAT¹  ADI PANCORO² 

[1] Department of Biology Education, Faculty of Mathematic and Science Education, Indonesia University of Education, Jalan Dr. Setiabudi 229, Bandung 40154, Indonesia;School of Life Science and Technology, Bandung Institute of Technology, Jalan Ganesha 10, Bandung 40132, Indonesia;

关键词: homogenous sequences;    internal transcribed spacer region;    molecular phylogenetic;    SSCP method;   

DOI  :  10.4308/hjb.17.1.50

来源: DOAJ

【 摘 要 】

In molecular phylogenetic study, homogeneity of DNA sequences is a prerequisite before putting it into practice. Internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) has been common in phylogenetic study, but a homogenous sequence is often difficult to obtain. Here we use single-stranded conformation polymorphism (SSCP) method to detect homogeneity for nine pooled amplified products of ITS region. Our results suggested that SSCP method has been applicable in detection homogeneity of ITS region prior to using it in sequencing processes.

【 授权许可】

Unknown