In Vitro Bioactivity and Biocompatibility of Bio-Inspired Ti-6Al-4V Alloy Surfaces Modified by Combined Laser Micro/Nano Structuring

Molecules

Zhen Shi¹  Chen Li¹  Lijun Yang¹  Pengfei Yang²  Guanghua Cheng³  Yong Yang⁴ 

[1] College of Mechanical and Electrical Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China;Key Laboratory of Space Radiobiology of Gansu Province, Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Institute of Modern Physics, CAS, Lanzhou 730000, China;School of Electronics and Information, Northwestern Polytechnical University, Xi’an 710072, China;State Key Laboratory of Transient Optics and Photonics, Xi’an Institute of Optics and Precision Mechanics, CAS, Xi’an 710119, China;

关键词: bioinspired surfaces;    bioactivity;    biocompatibility;    femtosecond laser;    lipss;    cell alignment;    cell adherence;    ti-6al-4v;   

DOI  :  10.3390/molecules25071494

来源: DOAJ

【 摘 要 】

The bioactivity and biocompatibility play key roles in the success of dental and orthopaedic implants. Although most commercial implant systems use various surface microstructures, the ideal multi-scale topographies capable of controlling osteointegration have not yielded conclusive results. Inspired by both the isotropic adhesion of the skin structures in tree frog toe pads and the anisotropic adhesion of the corrugated ridges on the scales of Morpho butterfly wings, composite micro/nano-structures, including the array of micro-hexagons and oriented nano-ripples on titanium alloy implants, were respectively fabricated by microsecond laser direct writing and femtosecond laser-induced periodic surface structures, to improve cell adherence, alignment and proliferation on implants. The main differences in both the bioactivity in simulated body fluid and the biocompatibility in osteoblastic cell MC3T3 proliferation were measured and analyzed among Ti-6Al-4V samples with smooth surface, micro-hexagons and composite micro/nano-structures, respectively. Of note, bioinspired micro/nano-structures displayed the best bioactivity and biocompatibility after in vitro experiments, and meanwhile, the nano-ripples were able to induce cellular alignment within the micro-hexagons. The reasons for these differences were found in the topographical cues. An innovative functionalization strategy of controlling the osteointegration on titanium alloy implants is proposed using the composite micro/nano-structures, which is meaningful in various regenerative medicine applications and implant fields.

【 授权许可】

Unknown