Reconstructing each cell’s genome within complex microbial communities - dream or reality?

Frontiers in Microbiology

Patrick eSchwientek¹  Christian eRinke¹  Tanja eWoyke¹  Alicia eClum¹  Scott eClingenpeel¹ 

[1] DOE Joint Genome Institute;

关键词: Environmental Microbiology;    microbial ecology;    Multiple Displacement Amplification;    single-cell sequencing;    genome completeness;    genome quality;   

DOI  :  10.3389/fmicb.2014.00771

来源: DOAJ

【 摘 要 】

As the vast majority of microorganisms have yet to be cultivated in a laboratory setting, access to their genetic makeup has largely been limited to cultivation-independent methods.These methods, namely metagenomics and more recently single-cell genomics, have become cornerstones for microbial ecology and environmental microbiology.One ultimate goal is the recovery of genome sequences from each cell within an environment to move towards a better understanding of community metabolic potential and to provide substrate for experimental work.As single-cell sequencing has the ability to decipher all sequence information contained in an individual cell, this method holds great promise in tackling such challenge.Methodological limitations and inherent biases however do exist, which will be discussed here as based on environmental and benchmark data, to assess on how far we are from reaching this goal.

【 授权许可】

Unknown