Physiological and Transcriptome Analyses Reveal Short-Term Responses and Formation of Memory Under Drought Stress in Rice

Frontiers in Genetics

Haoju Liu¹  Aizhong Liu²  Li Liu²  Lu Wang²  Jinyong Hu²  Andan Zhu²  Chengjun Zhang²  Heqiang Huo³  Yongjun Lin⁴  Ping Li⁵  Hong Yang⁵ 

[1] Department of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China;Key Laboratory for Economic Plants and Biotechnology, Germplasm Bank of Wild Species, Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory for Wild Plant Resources, Kunming, China;Mid-Florida Research and Education Center, Department of Environmental Horticulture, University of Florida, Gainesville, FL, United States;National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, China;University of Chinese Academy of Sciences, Beijing, China;

关键词: ssRNA-Seq;    short-term drought memory;    ABA;    photosynthesis;    proline;    memory factors;   

DOI  :  10.3389/fgene.2019.00055

来源: DOAJ

【 摘 要 】

In some plants, exposure to stress can induce a memory response, which appears to play an important role in adaptation to recurrent stress environments. However, whether rice exhibits drought stress memory and the molecular mechanisms that might underlie this process have remained unclear. Here, we ensured that rice drought memory was established after cycles of mild drought and re-watering treatment, and studied gene expression by whole-transcriptome strand-specific RNA sequencing (ssRNA-seq). We detected 6,885 transcripts and 238 lncRNAs involved in the drought memory response, grouped into 16 distinct patterns. Notably, the identified genes of dosage memory generally did not respond to the initial drought treatment. Our results demonstrate that stress memory can be developed in rice under appropriate water deficient stress, and lncRNA, DNA methylation and endogenous phytohormones (especially abscisic acid) participate in rice short-term drought memory, possibly acting as memory factors to activate drought-related memory transcripts in pathways such as photosynthesis and proline biosynthesis, to respond to the subsequent stresses.

【 授权许可】

Unknown