Oxytocin-Selective Nanogel Antibody Mimics

International Journal of Molecular Sciences

Teodor Aastrup¹  Börje Sellergren²  Rashmi Mahajan³  Ian A. Nicholls³  Subramanian Suriyanarayanan³  Gustaf D. Olsson³  Jesper G. Wiklander³ 

[1] Attana AB, Greta Arwidssons Väg 21, 11419 Stockholm, Sweden;Biofilms Research Center for Biointerfaces, Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 20506 Malmö, Sweden;Bioorganic and Biophysical Chemistry Laboratory, Linnaeus University Centre for Biomaterials Chemistry, Department of Chemistry and Biomedical Sciences, Linnaeus University, 39182 Kalmar, Sweden;

关键词: molecular dynamics;    molecularly imprinted polymer;    nanoparticle;    NMR;    peptide imprinting;    plastic antibody;   

DOI  :  10.3390/ijms23052534

来源: DOAJ

【 摘 要 】

Oxytocin imprinted polymer nanoparticles were synthesized by glass bead supported solid phase synthesis, with NMR and molecular dynamics studies used to investigate monomer–template interactions. The nanoparticles were characterized by dynamic light scattering, scanning- and transmission electron microscopy and X-ray photoelectron spectroscopy. Investigation of nanoparticle-template recognition using quartz crystal microbalance-based studies revealed sub-nanomolar affinity, k_d ≈ 0.3 ± 0.02 nM (standard error of the mean), comparable to that of commercial polyclonal antibodies, k_d ≈ 0.02–0.2 nM.

【 授权许可】

Unknown