Altered distribution of plasma PAF-AH between HDLs and other lipoproteins in hyperlipidemia and diabetes mellitus

Journal of Lipid Research

Tohru Egashira¹  Mayumi Ito¹  Jun Ishi²  Tadao Iwasaki²  Makoto Nagano³  Irina P. Stepanova³  Akito Kawaguchi⁴  Norman E. Miller⁴  Mitsuaki Ishihara⁵  Masahiro Tsuji⁵  Sadao Takahashi⁶  Hiroaki Hattori⁶  Takeshi Kujiraoka⁶ 

[1] Division of Internal Medicine, Hokkaido Hospital for Social Health Insurance, Sapporo, Japan;Graduate School of Education, Hokkaido University, Sapporo, Japan;Institute of Medical Science, Health Sciences University of Hokkaido, Sapporo, Japan;Molecular Pathology Laboratory, International Center for Genetic Engineering and Biotechnology, Trieste, Italy;Third Department of Internal Medicine, Fukui Medical University, Fukui, Japan;Department of Advanced Medical Technology and Development, BML, Inc., Saitama, Japan;

关键词: platelet-activating factor acetylhydrolase;    high density lipoprotein;    low density lipoprotein;    lipoprotein-associated phospholipase A2;    ELISA;    Invader® assay;   

DOI  :  

来源: DOAJ

【 摘 要 】

Platelet-activating factor acetylhydrolase (PAF-AH) is a phospholipase A2 associated with lipoproteins that hydrolyzes platelet-activating factor (PAF) and oxidized phospholipids. We have developed an ELISA for PAF-AH that is more sensitive than previous methods, and have quantified HDL-associated and non-HDL-associated PAF-AH in healthy, hyperlipidemic, and diabetic subjects. In healthy subjects, plasma total PAF-AH concentration was positively correlated with PAF-AH activity and with plasma total cholesterol, triacylglycerol, LDL cholesterol and apolipoprotein B (apoB) concentrations (all P < 0.01). HDL-associated PAF-AH concentration was correlated positively with plasma apoA-I and HDL cholesterol. Subjects with hyperlipidemia (n = 73) and diabetes mellitus (n = 87) had higher HDL-associated PAF-AH concentrations than did controls (P < 0.01). Non-HDL-associated PAF-AH concentration was lower in diabetic subjects than in controls (P < 0.01). Both hyperlipidemic and diabetic subjects had lower ratios of PAF-AH to apoB (P < 0.01) and higher ratios of PAF-AH to apoA-I (P < 0.01) than did controls.Our results show that the distribution of PAF-AH mass between HDLs and LDLs is determined partly by the concentrations of the lipoproteins and partly by the mass of enzyme per lipoprotein particle, which is disturbed in hyperlipidemia and diabetes mellitus.

【 授权许可】

Unknown