Inactivation Activities of Ozonated Water, Slightly Acidic Electrolyzed Water and Ethanol against SARS-CoV-2

Molecules

Hourei Oh¹  Yoshimasa Makita²  Akihiro Kaneko³  Sachiko Matsuda⁴  Haruko Ogawa⁴  Dulamjav Jamsransuren⁴  Yohei Takeda⁵ 

[1] Center of Innovation in Dental Education, Osaka Dental University, 8-1 Kuzuha Hanazono Hirakata, Osaka 573-1121, Japan;Department of Chemistry, Osaka Dental University, 8-1 Kuzuha Hanazono Hirakata, Osaka 573-1121, Japan;Department of Oral Surgery, Ikegami General Hospital, 6-1-19 Ikegami Ootaku, Tokyo 146-8531, Japan;Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro 080-8555, Japan;Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro 080-8555, Japan;

关键词: disinfectants;    ethanol;    hand hygiene;    ozonated water;    SARS-CoV-2;    slightly acidic electrolyzed water;   

DOI  :  10.3390/molecules26185465

来源: DOAJ

【 摘 要 】

This study aimed to compare the SARS-CoV-2-inactivation activity and virucidal mechanisms of ozonated water (OW) with those of slightly acidic electrolyzed water (SAEW) and 70% ethanol (EtOH). SARS-CoV-2-inactivation activity was evaluated in a virus solution containing 1%, 20% or 40% fetal bovine serum (FBS) with OW, SAEW or EtOH at a virus-to-test solution ratio of 1:9, 1:19 or 1:99 for a reaction time of 20 s. EtOH showed the strongest virucidal activity, followed by SAEW and OW. Even though EtOH potently inactivated the virus despite the 40% FBS concentration, virus inactivation by OW and SAEW decreased in proportion to the increase in FBS concentration. Nevertheless, OW and SAEW showed potent virucidal activity with 40% FBS at a virus-to-test solution ratio of 1:99. Real-time PCR targeting the viral genome revealed that cycle threshold values in the OW and SAEW groups were significantly higher than those in the control group, suggesting that OW and SAEW disrupted the viral genome. Western blotting analysis targeting the recombinant viral spike protein S1 subunit showed a change in the specific band into a ladder upon treatment with OW and SAEW. OW and SAEW may cause conformational changes in the S1 subunit of the SARS-CoV-2 spike protein.

【 授权许可】

Unknown