Self-Assembled Permanent Micro-Magnets in a Polymer-Based Microfluidic Device for Magnetic Cell Sorting

Cells

Emmanuelle Laurenceau¹  Jordyn Howard²  Samir Mekkaoui²  Lucie Descamps²  Marie-Charlotte Audry²  Anne-Laure Deman²  Clément Albin³  Damien Le Roy³  David Barthelemy⁴  Léa Payen⁴  Jessica Garcia⁴ 

[1] CNRS, INSA Lyon, CPE Lyon, CNRS, INL, UMR5270, University Lyon, Ecole Centrale de Lyon, Université Claude Bernard Lyon 1, 69130 Ecully, France;CNRS, INSA Lyon, Ecole Centrale de Lyon, CPE Lyon, INL, UMR5270, University Lyon, Université Claude Bernard Lyon 1, 69622 Villeurbanne, France;CNRS, UMR5306 Institut Lumière Matière, University Lyon, Université Claude Bernard Lyon 1, 69100 Villeurbanne, France;Laboratoire de Biochimie Et Biologie Moléculaire, Groupe Hospitalier Sud, Hospices Civils de Lyon, 69495 Pierre Bénite, France;

关键词: magnetophoresis;    micro-magnets;    polymer composite;    microfluidic devices;    particle separation;   

DOI  :  10.3390/cells10071734

来源: DOAJ

【 摘 要 】

Magnetophoresis-based microfluidic devices offer simple and reliable manipulation of micro-scale objects and provide a large panel of applications, from selective trapping to high-throughput sorting. However, the fabrication and integration of micro-scale magnets in microsystems involve complex and expensive processes. Here we report on an inexpensive and easy-to-handle fabrication process of micrometer-scale permanent magnets, based on the self-organization of NdFeB particles in a polymer matrix (polydimethylsiloxane, PDMS). A study of the inner structure by X-ray tomography revealed a chain-like organization of the particles leading to an array of hard magnetic microstructures with a mean diameter of 4 µm. The magnetic performance of the self-assembled micro-magnets was first estimated by COMSOL simulations. The micro-magnets were then integrated into a microfluidic device where they act as micro-traps. The magnetic forces exerted by the micro-magnets on superparamagnetic beads were measured by colloidal probe atomic force microscopy (AFM) and in operando in the microfluidic system. Forces as high as several nanonewtons were reached. Adding an external millimeter-sized magnet allowed target magnetization and the interaction range to be increased. Then, the integrated micro-magnets were used to study the magnetophoretic trapping efficiency of magnetic beads, providing efficiencies of 100% at 0.5 mL/h and 75% at 1 mL/h. Finally, the micro-magnets were implemented for cell sorting by performing white blood cell depletion.

【 授权许可】

Unknown