Imaging of redox-imbalance and oxidative stress in kidney in vivo, induced by dietary cholesterol

Biotechnology & Biotechnological Equipment

Itsuko Ishii¹  Ichio Aoki²  Sayaka Shibata²  Rumiana Bakalova³  Dessislava Lazarova³  Genoveva Zlateva³  Zhivko Zhelev⁴ 

[1] Chiba University;National Institute of Radiological Sciences (QST-NIRS);Sofia University “St. Kliment Ohridski”;Trakia University;

关键词: hypercholesterolemia;    kidney dysfunction;    redox-imbalance;    oxidative stress;    magnetic resonance imaging;    cyclic nitroxides;   

DOI  :  10.1080/13102818.2019.1573153

来源: DOAJ

【 摘 要 】

We used a mitochondria-penetrating nitroxide, mito-TEMPO, as a contrast probe for imaging of kidney dysfunction in mice, based on the redox-imbalance and oxidative stress in the renal tissues. Kidney dysfunction was triggered by hypercholesterolemia. The mice were divided in three groups: (i) on normal diet (ND; control); (ii) on cholesterol diet (CD); (iii) on cholesterol plus cholestyramine diet (CC). CD mice showed increased plasma levels of total cholesterol and non-HDL-cholesterol, as well as increased serum levels of blood urea nitrogen, uric acid and creatinine, compared to ND mice. CC mice showed slightly increased plasma levels of total cholesterol and HDL-cholesterol, but not non-HDL-cholesterol, compared to ND mice. The serum levels of blood urea nitrogen, uric acid and creatinine in CC mice were equal to those in ND mice. The MRI signal of mito-TEMPO in the kidneys was characterized by: high intensity and long life-time in CD mice, indicating a high oxidative capacity of renal tissues; poor intensity and short life-time in ND mice, indicating a high reducing capacity of renal tissues; moderate intensity and relatively short life-time in CC mice, which shows the protective effect of lipid-lowering agents against oxidative damage. The data suggest that hypercholesterolemia induces redox-imbalance and oxidative stress in kidneys and this process could be visualized using MRI and mito-TEMPO as a redox-sensitive contrast substance.

【 授权许可】

Unknown