Differential Effects of Viscum album Preparations on the Maturation and Activation of Human Dendritic Cells and CD4+ T Cell Responses

Molecules

Srini V. Kaveri¹  Emmanuel Stephen-Victor¹  Mrinmoy Das¹  Chaitrali Saha¹  Jagadeesh Bayry¹  Alain Friboulet² 

[1] Institut National de la Santé et de la Recherche Médicale Unité 1138, Centre de Recherche des Cordeliers, 15 rue de l’Ecole de Médicine, Paris F-75006, France;Université de Technologie de Compiègne, UMR CNRS 6022, Compiègne F-60205, France;

关键词: Viscum album;    innate cells;    dendritic cells;    maturation;    cytokines;    T cell response;    IFN-γ;    Th17;    Th1;    Th2;    regulatory T cell;   

DOI  :  10.3390/molecules21070912

来源: DOAJ

【 摘 要 】

Extracts of Viscum album (VA); a semi-parasitic plant, are frequently used in the complementary therapy of cancer and other immunological disorders. Various reports show that VA modulates immune system and exerts immune-adjuvant activities that might influence tumor regression. Currently, several therapeutic preparations of VA are available and hence an insight into the mechanisms of action of different VA preparations is necessary. In the present study, we performed a comparative study of five different preparations of VA on maturation and activation of human dendritic cells (DCs) and ensuing CD4+ T cell responses. Monocyte-derived human DCs were treated with VA Qu Spez, VA Qu Frf, VA M Spez, VA P and VA A. Among the five VA preparations tested VA Qu Spez, a fermented extract with a high level of lectins, significantly induced DC maturation markers CD83, CD40, HLA-DR and CD86, and secretion of pro-inflammatory cytokines such as IL-6, IL-8, IL-12 and TNF-α. Furthermore, analysis of T cell cytokines in DC-T cell co-culture revealed that VA Qu Spez significantly stimulated IFN-γ secretion without modulating regulatory T cells and other CD4+ T cytokines IL-4, IL-13 and IL-17A. Our study thus delineates differential effects of VA preparations on DC maturation; function and T cell responses.

【 授权许可】

Unknown