Extracellular Vesicles: Current Analytical Techniques for Detection and Quantification

Biomolecules

María Carmen Blanco-López¹  Amanda Moyano¹  Myriam Oliveira-Rodríguez¹  Esther Serrano-Pertierra¹  Montserrat Rivas²  María Salvador²  María Matos³  Gemma Gutiérrez³ 

[1] Department of Physical and Analytical Chemistry, University of Oviedo, 33006 Oviedo, Spain;Department of Physics & IUTA, University of Oviedo, Campus de Viesques, 33204 Gijón, Spain;Instituto Universitario de Biotecnología de Asturias, University of Oviedo, 33006 Oviedo, Spain;

关键词: extracellular vesicles;    characterization;    quantification;    ELISA;    lateral flow immunoassay;    nanoparticle tracking analysis;   

DOI  :  10.3390/biom10060824

来源: DOAJ

【 摘 要 】

Since their first observation, understanding the biology of extracellular vesicles (EV) has been an important and challenging field of study. They play a key role in the intercellular communication and are involved in important physiological and pathological functions. Therefore, EV are considered as potential biomarkers for diagnosis, prognosis, and monitoring the response to treatment in some diseases. In addition, due to their properties, EV may be used for therapeutic purposes. In the study of EV, three major points have to be addressed: 1. How to isolate EV from cell culture supernatant/biological fluids, 2. how to detect them, and 3. how to characterize and quantify. In this review, we focus on the last two questions and provide the main analytical techniques up-to-date for detection and profiling of EV. We critically analyze the advantages and disadvantages of each one, aimed to be of relevance for all researchers working on EV biology and their potential applications.

【 授权许可】

Unknown