期刊论文详细信息
Biology | |
In Vitro High Throughput Screening, What Next? Lessons from the Screening for Aurora Kinase Inhibitors | |
Ly-Thuy-Tram Le1  Thi-My-Nhung Hoang1  Hong-Lien Vu1  Annie Molla1  Chi-Hung Nguyen2  | |
[1] INSERM UJF U823 Institut Albert Bonniot, Team 4: Chromatin and Epigenetic, BP 170,38 042 Grenoble Cedex 9, France;UMR 176 CNRS-Institut Curie, Bat 110 Centre Universitaire, Orsay 91405, France; | |
关键词: aurora; kinase; kinase inhibitor; Gck; Nuak1; Tak-1; | |
DOI : 10.3390/biology3010167 | |
来源: DOAJ |
【 摘 要 】
Based on in vitro assays, we performed a High Throughput Screening (HTS) to identify kinase inhibitors among 10,000 small chemical compounds. In this didactic paper, we describe step-by-step the approach to validate the hits as well as the major pitfalls encountered in the development of active molecules. We propose a decision tree that could be adapted to most in vitro HTS.
【 授权许可】
Unknown