A Fosmid-Based System for the Generation of Recombinant Cercopithecine Alphaherpesvirus 2 Encoding Reporter Genes

Viruses

Marie Wende¹  Stefan Pöhlmann¹  Alexander Mehr¹  Abdul Rahman Siregar¹  Michael Winkler¹  Ekaterina Chukhno¹  Sabine Gärtner¹  Jasper Götting²  Akshay Dhingra²  Thomas Schulz²  Stoyan Petkov³ 

[1] Infection Biology Unit, German Primate Center-Leibniz Institute for Primate Research, 37077 Göttingen, Germany;Institute of Virology, Hannover Medical School, 30625 Hannover, Germany;Platform Degenerative Diseases, German Primate Center-Leibniz Institute for Primate Research, 37077 Göttingen, Germany;

关键词: cercopithecine alphaherpesvirus 2;    fosmid;    recombineering;   

DOI  :  10.3390/v11111026

来源: DOAJ

【 摘 要 】

The transmission of Macacine alphaherpesvirus 1 (McHV-1) from macaques, the natural host, to humans causes encephalitis. In contrast, human infection with Cercopithecine alphaherpesvirus 2 (CeHV-2), a closely related alphaherpesvirus from African vervet monkeys and baboons, has not been reported and it is believed that CeHV-2 is apathogenic in humans. The reasons for the differential neurovirulence of McHV-1 and CeHV-2 have not been explored on a molecular level, in part due to the absence of systems for the production of recombinant viruses. Here, we report the generation of a fosmid-based system for rescue of recombinant CeHV-2. Moreover, we show that, in this system, recombineering can be used to equip CeHV-2 with reporter genes. The recombinant CeHV-2 viruses replicated with the same efficiency as uncloned, wt virus and allowed the identification of cell lines that are highly susceptible to CeHV-2 infection. Collectively, we report a system that allows rescue and genetic modification of CeHV-2 and likely other alphaherpesviruses. This system should aid future analysis of CeHV-2 biology.

【 授权许可】

Unknown