Coupling (reduced) Graphene Oxide to Mammalian Primary Cortical Neurons In Vitro

AIMS Materials Science

Andrew M. H. Ng¹  Milos Nesládek²  Farnoosh Vahidpour³  Michele Giugliano³  Kian Ping Loh⁴  Antonina M. Monaco⁵  Jaroslaw Motylewski⁵  Anastasiya Moskalyuk⁵ 

[1] IMOMEC associated laboratory, IMEC, Kapeldreef 75, 3001 Leuven, Belgiu;Department of Chemistry and Graphene Research Centre, National University of Singapore, 3 Science Drive 3, Singapore 11754;Institute for Materials Research, Material Physics Division, Hasselt University, 3590 Diepenbeek, Belgium;Institute of Materials Research and Engineering, Agency for Science, Technology and Research, 3 Research Link, Singapore 11760;Theoretical Neurobiology and Neuroengineering Laboratory, Dept. of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk, Belgiu;

关键词: graphene oxide;    neuroengineering;    primary neuronal cultures;    cellular electrophysiology;    cytotoxicity;   

DOI  :  10.3934/matersci.2015.3.217

来源: DOAJ

【 摘 要 】

Neuronal nanoscale interfacing aims at identifying or designing nanostructured smart materials and validating their applications as novel biocompatible scaffolds with active properties for neuronal networks formation, nerve regeneration, and bidirectional biosignal coupling. Among several carbon-based nanomaterials, Graphene recently attracted great interest for biological applications, given its unique mechanical, optical, electronic properties, and its recent technological applications. Here we explore the use of Graphene Oxide (GO) and reduced Graphene Oxide (rGO) as biocompatible culture substrates for primary neuronal networks developing ex vivo. We quantitatively studied cytotoxicity and cellular viability as well as single-cell and network-level electrophysiological properties of neurons in vitro. Our results confirm previous reports, employing immortalized cell lines or pluripotent stem cells, and extend them to mammalian primary cortical neurons: GO and rGO are biocompatible substrates and do not alter neuronal excitable properties.

【 授权许可】

Unknown