Vitreous microparticles contain apoptotic signals suggesting a diabetic vitreopathy

International Journal of Ophthalmology

Michael A Paley¹  Albert S. Li²  Kisha Deslee Piggott³  Rajendra Shridhar Apte³  Brigid Marshall³  Rithwick Rajagopal³  Prabakar Kumar Rao³  Lynn Marisa Hassman³  Harris Sultan⁴ 

[1] Department of Medicine, Division of Rheumatology, Washington University, St. Louis, MO 63110, USA;Department of Ophthalmology and Visual Sciences, University of Maryland School of Medicine, Baltimore, MD 21201, USA;John Hardesty Department of Ophthalmology, Washington University, St. Louis, MO 63110, USA;Rajendra Shridhar Apte. John Hardesty Department of Ophthalmology, Washington University, 660 South Euclid Avenue, Campus Box 8096, St. Louis, MO 63110, USA. [e-mail];

关键词: cell-derived microparticles;    vitrectomy;    vitreous;    diabetes mellitus;    apoptosis;   

DOI  :  10.18240/ijo.2022.01.14

来源: DOAJ

【 摘 要 】

AIM: To evaluate differences in microparticle profiles in vitreous samples between diabetic and non-diabetic eyes undergoing vitrectomy. METHODS: Un-masked cross-sectional series of 34 eyes undergoing vitrectomy. Vitreous specimens were collected and processed to evaluate for membrane integrity (DAPI), apoptosis (Annexin-V), and endothelial-cell origin (V-Cadherin). A BD LSR II flow cytometer was used for analysis and standardized sub-micron-sized beads were used for size comparison. RESULTS: Thirty-four specimens underwent analysis. Greater levels of Annexin-V were found on microparticles from specimens in which blood had entered the vitreous (n=12) compared to those without blood (n=22; 52.3%±30.7% vs 19.6%±27.2%, P=0.002). Patients with diabetes having surgery with hemorrhage (n=7) had greater expression of Annexin-V than those without hemorrhage (n=8; 62.1%±31.7% vs 18.9%±20.9%, P=0.009). However, in patients with non-diabetic vitreous hemorrhage, the level of Annexin-V expression was not significantly different compared to other disease processes (38.6%±25.7%, n=5 vs 20.0%±30.9%, n=14, P=0.087). CONCLUSION: Increased expression of the apoptotic marker, Annexin-V is detected on vitreous microparticles in diabetes-related vitreous hemorrhage. When evaluating vitreous hemorrhage in patients without diabetes, the apoptotic signal is not significantly different. Vitrectomy in patients with diabetes, and improvement in visual outcomes, may be related to the removal of a serum-derived, pro-apoptotic vitreous. Further investigation is warranted in order to identify the molecular characteristics of microparticles that regulate disease.

【 授权许可】

Unknown