In vitro and pilot in vivo imaging of 18 kDa translocator protein (TSPO) in inflammatory vascular disease

EJNMMI Research

Nicole Barthe¹  Anne Rullier²  Robert B. Innis³  Yasuyuki Kimura³  Masahiro Fujita³  Andrea Zhang³  Paolo Zanotti-Fregonara³  Igor Sibon⁴  Romain Schollhammer⁵  Clément Morgat⁵  Sébastien Lepreux⁶  Delphine Vimont⁷  Xavier Berard⁸ 

[1] BioTis;Histologic Department, University Hospital of Bordeaux;Molecular Imaging Branch, NIMH;Neurology Department, University Hospital of Bordeaux;Nuclear Medicine Department, University Hospital of Bordeaux;Pathology Unit, CH de Libourne;University of Bordeaux, INCIA, UMR5287;Vascular Surgery Department, University Hospital of Bordeaux;

关键词: TSPO;    PET/CT;    Inflammation;    Atherosclerosis;    Vasculitis;   

DOI  :  10.1186/s13550-021-00786-7

来源: DOAJ

【 摘 要 】

Abstract Background Inflammatory vascular disease of the arteries, such as inflamed atheromatous plaques or arteritis, may cause aneurysms or ischemic strokes. In this context, using positron emission tomography (PET) to image inflammation may help select patients who would benefit from appropriate therapeutic interventions. This study sought to assess the usefulness of the 18 kDa translocator protein (TSPO) tracers [11C]-PBR28 and [18F]-PBR06 for imaging inflammatory vascular disease in vitro and in vivo. Immunohistochemistry for macrophage infiltration as well as autoradiography with [18F]-PBR06 were performed on eight paraffin-embedded, formalin-fixed atherosclerosis plaques prospectively collected after carotid endarterectomy of eight patients affected by ischemic stroke. Six different patients, one of whom was also included in the in vitro study, underwent PET imaging. Two patients with carotid stenosis associated with ischemic stroke were imaged with [18F]-PBR06 PET/CT, and four other patients (three with large vessel vasculitis and one with bilateral carotid stenosis but without stroke) were imaged with [11C]-PBR28. Results All in vitro sections showed specific binding of [18F]-PBR06, which co-localized with immunohistochemistry markers for inflammation. However, in vivo TSPO imaging with either [11C]-PBR28 or [18F]-PBR06 was negative in all participants. Conclusion Despite good uptake on surgical samples in vitro, [11C]-PBR28 and [18F]-PBR06 are not viable clinical tools for imaging inflammatory vascular disease. Trial registration: NCT02513589, registered 31 July 2015 and NCT00547976, registered 23 October 2007. https://clinicaltrials.gov .

【 授权许可】

Unknown