Improved Urothelial Cell Proliferation, Cytoskeleton and Barrier Function Protein Expression in the Patients With Interstitial Cystitis/Bladder Pain Syndrome After Intravesical Platelet-Rich Plasma Injection

International Neurourology Journal

Teng-Yi Lin¹  Han-Chen Ho²  Yung-Hsiang Hsu³  Jia-Fong Jhang⁴  Yuan-Hong Jiang⁴  Hann-Chorng Kuo⁴  Lori A Birder⁵ 

[1] Department Laboratory Medicine, Hualien Tzu Chi Hospital and Buddhist Tzu Chi Medical Foundation, Buddhist Tzu Chi University, Hualien, Taiwan;Department of Anatomy, Tzu Chi University, Hualien, Taiwan;Department of Pathology, Hualien Tzu Chi Hospital, Tzu Chi Medical Foundation, and Tzu Chi University, Hualien, Taiwan;Department of Urology, Hualien Tzu Chi Hospital, Tzu Chi Medical Foundation, and Tzu Chi University, Hualien, Taiwan;Departments of Medicine, Pharmacology, and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA;

关键词: urothelium;    pathogenesis;    human;    platelet;    bladder;   

DOI  :  10.5213/inj.2142100.050

来源: DOAJ

【 摘 要 】

Purpose To investigate urothelial cell proliferation, cytoskeleton, inflammation, and barrier function protein expressions in patients with interstitial cystitis/bladder pain syndrome (IC/BPS) after intravesical platelet-rich plasma (PRP) injections Methods A total of 19 patients with IC/BPS underwent 4 monthly intravesical PRP injections. Bladder biopsies were taken at the first and fourth PRP treatment. The bladder specimens were analyzed using the Western blot and immunochemical staining for progenitor cell markers for sonic hedgehog (Shh), CD34, and cytoskeleton proteins cytokeratin 5 (CK5), CK14, CK20; barrier function markers for zonula occludens-1 (ZO-1), E-cadherin, and intercellular adhesive molecule-1, tryptase and transforming growth factor-β (TGF-β). Global response assessment (GRA) was used to evaluate treatment outcomes. Results The mean age of patients was 55.6 years. After PRP injections, the functional bladder capacity and maximum flow rate increased, and the visual analogue scale (VAS) of pain, interstitial cystitis (IC) symptom index, IC problem index, O’Leary-Sant symptom score, and GRA improved in all patients. Urothelium Shh, CK5, ZO-1, E-cadherin, and TGF-β expressions increased significantly after repeated PRP injections. By subgrouping, according to PRP treatment outcomes, significant increases in Shh, E-cadherin, and ZO-1 expressions were noted only in patients with GRA ≥1 or improved VAS, but not in patients with GRA=0 and no improvement in VAS. Conclusions The level of urothelial barrier function protein and cell proliferation protein expression in the patients with IC/BPS was increased after repeat intravesical PRP injections. Intravesical repeat PRP injections may have potential to improve urothelial health and result in symptoms improvement in the patients with IC/BPS.

【 授权许可】

Unknown