Genome-Wide Identification of Long Non-coding RNAs in the Gravid Ectoparasite Varroa destructor

Frontiers in Genetics

Zheguang Lin¹  Hao Xu¹  Ting Ji¹  Xiaomei Chen¹  Guohong Chen¹  Cong Han¹  Yibing Liu¹  Heng Chen¹  Xiaoling Su²  Yujiao Li³  Yalu Ke⁴  Wei Wang⁴ 

[1] Apicultural Research Institute, College of Animal Science and Technology, Yangzhou University, Yangzhou, China;Jinhua Academy of Agricultural Sciences, Jinhua, China;Shandong Apiculture Breeding of Improved Varieties and Extension Center, Tai’an, China;Wuzhong Animal Health Supervision Institute, Suzhou, China;

关键词: Varroa destructor;    long non-coding RNA;    honey bee;    ectoparasitic mite;    oogenesis;   

DOI  :  10.3389/fgene.2020.575680

来源: DOAJ

【 摘 要 】

Long non-coding RNAs (lncRNAs) emerge as critical regulators across a wide variety of biological functions in living organisms. However, to date, no systematic characterization of lncRNAs has been investigated in the ectoparasitic mite Varroa destructor, the most severe biotic threat to honey bees worldwide. Here, we performed an initial genome-wide identification of lncRNAs in V. destructor via high-throughput sequencing technology and reported, for the first time, the transcriptomic landscape of lncRNAs in the devastating parasite. By means of a lncRNA identification pipeline, 6,645 novel lncRNA transcripts, encoded by 3,897 gene loci, were identified, including 2,066 sense lncRNAs, 2,772 lincRNAs, and 1,807 lncNATs. Compared with protein-coding mRNAs, V. destructor lncRNAs are shorter in terms of full length, as well as of the ORF length, contain less exons, and express at lower level. GO term and KEGG pathway enrichment analyses of the lncRNA target genes demonstrated that these predicted lncRNAs may be potentially responsible for the regulatory functions of cellular and biological progresses in the reproductive phase of V. destructor. To our knowledge, this is the first catalog of lncRNA profile in the parasitiformes species, providing a valuable resource for genetic and genomic studies. Understanding the characteristics and features of lncRNAs in V. destructor would promote sustainable parasite control.

【 授权许可】

Unknown