The Sesquiterpene Biosynthesis and Vessel-Occlusion Formation in Stems of Aquilaria sinensis (Lour.) Gilg Trees Induced by Wounding Treatments without Variation of Microbial Communities

International Journal of Molecular Sciences

Hui Meng¹  Yun Yang¹  Jianhe Wei²  Yanhong Xu²  Zheng Zhang²  Zhihui Gao²  Xiaomin Han²  Liang Liang² 

[1] Hainan Provincial Key Laboratory of Resources Conservation and Development ofSouthern Medicine, Hainan Branch of the Institute of Medicinal Plant Development,Chinese Academy of Medicinal Sciences and Peking Union Medical College, Wanning 571533, China;National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences andPeking Union Medical College, Beijing 100193, China;

关键词: Aquilaria sinensis;    wound;    microbe;    vessel occlusion;    sesquiterpene;    plant defense response;   

DOI  :  10.3390/ijms151223589

来源: DOAJ

【 摘 要 】

As widely recognized, agarwood formation in Aquilaria trees is induced by external wounding. Because agarwood usually harbors specific microbes, the function of microbes in agarwood formation has been debated for almost a century. In this study, two wounding methods, the burning-chisel-drilling method (BCD) and the whole-tree agarwood-inducing method (Agar-Wit), were used under the non-contamination of environmental microorganisms. After pyrosequencing the small rRNA subunits of the wounds induced by the BCD andAgar-Wit, no substantial variation was observed either in fungal and bacterial enrichment and diversity or in the relative abundances of taxa. By contrast, significant variations in fungal and bacterial communities were detected following the partial tree pruning (PTP)-wounding. The wound-induced sesquiterpene biosynthesis and vessel-occlusion formation, however, were found to be similar in all types of wounded trunks. We thus infer that wounding in the absence of variations in microbial communities may induce agarwood formation. This result does not support the long-standing notion that agarwood formation depends on microbes.

【 授权许可】

Unknown