Water-Soluble Fiber from Bengkoang (Pachyrhizus erosus (L.) Urban) Tuber Modulates Immune System Activity in Male Mice

Scientia Pharmaceutica

Endang Lukitaningsih¹  AgungEndro Nugroho²  HanifNasiatul Baroroh²  Arief Nurrochmad² 

[1] Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281, Indonesia;Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281, Indonesia;

关键词: immunomodulatory effect;    Pachyrhizus erosus (L.) urban;    water-soluble fiber;    phagocytic activity;    adaptive immune;   

DOI  :  10.3390/scipharm88030034

来源: DOAJ

【 摘 要 】

Our previous study showed that water-soluble fiber from bengkoang (Pachyrizus erosus (L.) Urban) fiber extract (BFE) and bengkoang fiber fraction B (BFE-B) have phagocytic activity and modulation of cytokine production in vitro. The present study evaluates the immunomodulatory effects of water-soluble fibers BFE and BFE-B on male mice induced by hepatitis B vaccine. Thirty mice were divided into six groups and induced by hepatitis B vaccine intraperitoneally on days 7 and 14. The mice were then treated with BFE, BFE-B, levamisole, or sodium carboxymethyl cellulose for 18 days. At the end of the treatments (day 19), phagocytic activity, lymphocyte proliferation, spleen index, cytokine, and immunoglobulin G (IgG) production were determined. The results showed that the water-soluble fiber treatment could significantly increase phagocytic capacity, nitric oxide production, and spleen index. However, BFE-B could modulate tumor necrosis factor (TNF)-α and interleukin (IL)-10 secretion, BFE demonstrated no such effect on cytokine production. Lymphocyte proliferation assay revealed that treatment with 50 mg/kg body weight (BW) BFE and 50 mg/kg BW BFE-B could significantly enhance lymphocyte proliferation. Treatment with 25 and 50 mg/kg BW BFE-B stimulated IgG production. In conclusion, BFE and BFE-B similarly have immunomodulatory effects on innate immune responses. BFE-B further demonstrated immunomodulatory effects on adaptive immune responses.

【 授权许可】

Unknown