A Simple Graphene Functionalized Electrochemical Aptasensor for the Sensitive and Selective Detection of Glycated Albumin

Applied Sciences

Attawut Chaibunruang¹  Ratree Tavichakorntrakool¹  Nang Noon Shean Aye¹  Jureerut Daduang¹  Pornsuda Maraming¹  Prinya Prasongdee²  Patcharee Boonsiri³  Vittaya Amornkitbamrung⁴  Nattiya Teawtrakul⁵  Sakda Daduang⁶ 

[1] Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand;Clinical Chemistry Unit of Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;Department of Physics, Khon Kaen University, Khon Kaen 40002, Thailand;Division of Hematology, Department of Internal Medicine, Faculty of Medicine, Srinagarind Hospital, Khon Kaen University, Khon Kaen 40002, Thailand;Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand;

关键词: electrochemical aptasensor;    graphene oxide;    diabetes mellitus;    glycated albumin;   

DOI  :  10.3390/app112110315

来源: DOAJ

【 摘 要 】

Glycated albumin (GA) has been previously introduced as a promising biomarker for glycemic monitoring in diabetes patients with thalassemia. In this study, a label-free graphene oxide (GO)-modified aptasensor was developed for the rapid detection of GA. The fabrication of the aptasensor was dependent on the covalent interaction of the amine-functionalized GA-specific aptamer with the carboxylic groups of GO. Square wave voltammetry (SWV) analysis was carried out for the measurement of GA-aptamer binding to their specific proteins. The peak current changes before and after incubation with GA protein were directly proportional to the concentration. The developed aptasensor exhibited a broad linearity (1–10,000 µg mL⁻¹), a low detection limit (LOD) of 0.031 µg mL⁻¹, and high selectivity for GA detection. In addition, the aptasensor was successfully applied to detect GA in both spiked and clinical serum samples. The comparison of the developed method with a commercial assay validated the reliability of the aptasensor for clinical application. Therefore, the newly developed aptasensor is a promising tool for GA measurements in diabetic patients with underlying thalassemia.

【 授权许可】

Unknown