Genomic evidence supports the introgression between two sympatric stickleback species inhabiting the White Sea basin

Heliyon

Fedor Sharko¹  Eugenia Boulygina²  Artem Nedoluzhko³  Svetlana Tsygankova⁴  Amina Ibragimova⁵  Jorge Galindo-Villegas⁵  Anton Teslyuk⁵  Sergey Rastorguev⁵ 

[1] Corresponding author.;National Research Center “Kurchatov Institute”, 123182 Moscow, Russia;Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway;Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia;National Research Center “Kurchatov Institute”, 123182 Moscow, Russia;

关键词: Gasterosteus aculeatus;    Introgression;    Pungitius pungitius;    RAD-Seq;    Stickleback;    Transposable elements;   

DOI  :  

来源: DOAJ

【 摘 要 】

Interspecies hybridization is driven by a complex interplay of factors where introgression plays an important role. In the present study, the transfer of genetic material, between two quite distant fish species from different genera, through spontaneous hybridization was documented with dedicated molecular and bioinformatics tools. We investigate the genomic landscape of putative stickleback-relative introgression by carefully analyzing the tractable transposable elements (TE) on the admixed genome of some individuals of two sympatric stickleback species inhabiting northwestern Russia, namely the three-spined (Gasterosteus aculeatus) and the nine-spined (Pungitius pungitius) sticklebacks. Our data revealed that unique TE amplification types exist, supporting our proposed hypothesis that infers on the interspecific introgression. By running a restriction site-associated DNA sequencing (RAD-Seq) with eight samples of G. aculeatus and P. pungitius and subjecting further the results to a contrasting analysis by variated bioinformatic tools, we identified the related introgression-linked markers. The admixture nature observed in a single sample of the nine-spined stickleback demonstrated the possible traces of remote introgression between these two species. Our work reveals the potential that introgression has on providing particular variants at a high-frequency speed while linking blocks of sequence with multiple functional mutations. However, even though our results are of significant interest, an increased number of samples displaying the introgression are required to further ascertain our conclusions.

【 授权许可】

Unknown