Characterization of Human Tear Fluid by Means of Surface-Enhanced Raman Spectroscopy

Sensors

SalvatoreDel Prete¹  Mikhail Lisitskiy²  Carlo Camerlingo²  Marianna Portaccio³  Maria Lepore³  Daniela Montorio⁴  Gilda Cennamo⁵ 

[1] CISME—Centro Interdipartimentale di Microscopia Elettronica, Universitá di Napoli ’Federico II’, 80100 Naples, Italy;Consiglio Nazionale delle Ricerche, SPIN-CNR, Via Campi Flegrei 34, 80078 Pozzuoli, Italy;Dipartimento di Medicina Sperimentale, Università della Campania “L. Vanvitelli”, 80138 Naples, Italy;Dipt. di Neuroscienze e Scienze Riproduttive ed Odontostomatologiche, Universitá di Napoli ’Federico II’, 80121 Naples, Italy;Dipt. di Sanitá Pubblica, Universitá di Napoli ’Federico II’, 80131 Naples, Italy;

关键词: SERS;    tear;    biomedical sensors;    lactoferrin;    lysozyme;   

DOI  :  10.3390/s19051177

来源: DOAJ

【 摘 要 】

Tears are exceptionally rich sources of information on the health status of the eyes, as well as of whole body functionality, due to the presence of a large variety of salts and organic components whose concentration can be altered by pathologies, eye diseases and/or inflammatory processes. Surface enhanced Raman spectroscopy (SERS) provides a unique method for analyzing low concentrations of organic fluids such as tears. In this work, a home-made colloid of gold nanoparticles has been used for preparing glass substrates able to efficiently induce an SERS effect in fluid samples excited by a He–Ne laser ( λ= 633 nm). The method has been preliminary tested on Rhodamine 6G aqueous solutions at different concentrations, proving the possibility to sense substance concentrations as low as fewμ M, i.e., of the order of the main tear organic components. A clear SERS response has been obtained for human tear samples, allowing an interesting insight into tear composition. In particular, aspartic acid and glutamic acid have been shown to be possible markers for two important human tear components, i.e., lactoferrin and lysozyme.

【 授权许可】

Unknown