Experimental study regarding the effects of pulsed short waves on nervous tissue

Balneo Research Journal

CRACIUN Constantin¹  DOGARU Gabriela² 

[1] Babeş-Bolyai” University Cluj-Napoca, Center of Electron Microscopy;Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca;

关键词: pulsed short wave;    Diapulse;    nervous tissue;   

DOI  :  10.12680/balneo.2014.1063

来源: DOAJ

【 摘 要 】

Pulsed short waves are part of high frequency therapy and have through their biological effects, the good therapeutic results and the lack of side effects many indications in various disorders: rheumatologic, traumatologic, burns, neurological (nerve tissue regeneration, ischemic vascular accidents), vascular disorders. The experimental study consisted of the exposure of laboratory animals to the action of pulsed short waves in different doses and the monitoring of changes in the brain cellular and subcellular structure. The study included 35 Wistar rats, assigned to four groups, three groups of 10 animals and the control group of five non-irradiated animals. Group I was exposed to a dose of 1/80 cycles/sec, group II to 4/400 cycles/sec, and group III to 6/600 cycles/sec, for 10 minutes/day, for 15 days. After the rats were sacrificed, nervous tissue fragments were collected. These were adequately processed for their examination by optical and electron microscopy. At structural level, in the control group and the groups exposed to the dose of 1/80 cycles/sec, the neurons were normally structured. In group II, a slight increase of brain metabolic activity was seen, in group III there was a significant increase of brain metabolism, with better myelinated white matter axons, which suggests a better protection for the rapid conduction of nerve impulses to effectors. In group II, electron microscopy revealed similar aspects to those of the control group. A high ribosome density was found in the neuroplasm, which means an intense protein synthesis process. In group III, there was an intense protein synthesis activity, and a strong axon myelination in the white matter, resulting in a better protection for the transmission of nerve impulses. Conclusions: pulsed short waves caused brain changes depending on the dose. The analysis of electron microscopic images showed that irradiation at a dose of 6/600 cycles/sec induced an intense protein synthesis activity and a better axon myelination, which conferred a better protection for the transmission of impulses, more obvious than in the group irradiated at a dose of 4/400 cycles/sec.

【 授权许可】

Unknown