Serum miR-339-3p as a potential diagnostic marker for non-small cell lung cancer

Cancer Biology & Medicine

Sumonmal Uttayamakul¹  Keson Trakunram²  Pichitpon Chaniad²  Pritsana Raungrut²  Warangkana Keeratichananont³  Sarayut Lucien Geater³  Paramee Thongsuksai⁴  Voravit Chittithavorn⁵  Suhaimee Buya⁶ 

[1] Bamrasnaradura Infectious Diseases Institute, Nonthaburi 11000, Thailand;Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand;Department of Internal Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand;Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand;Department of Surgery, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand;Medical Data Center for Research and Innovation, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand;

关键词: biomarker;    diagnosis;    microrna;    non-small cell lung cancer;    quantitative real-time polymerase chain reaction;   

DOI  :  10.20892/j.issn.2095-3941.2020.0063

来源: DOAJ

【 摘 要 】

Objective: MicroRNA (miRNA), a short noncoding RNA, is claimed to be a potential blood-based biomarker. We aimed to identify and evaluate miRNAs as diagnostic biomarkers for non-small cell lung cancer (NSCLC). Methods: Profiles of 745 miRNAs were screened in the serum of 8 patients with NSCLC and 8 age- and sex-matched controls using TaqMan low-density arrays (TLDAs) and validated in 25 patients with NSCLC and 30 with other lung diseases (OLs) as well as in 19 healthy persons (HPs). The diagnostic performance of the candidate miRNAs was assessed in 117 cases of NSCLC and 113 OLs using quantitative real-time polymerase chain reaction (qRT-PCR). Differences in miRNA expression between patients with NSCLC and controls were assessed using the Mann–Whitney U test. The area under receiver operating characteristic (ROC) curve (AUC) was obtained based on the logistic regression model. Results: Ten miRNAs were found to be differentially expressed between patients with NSCLC and controls, including miR-769, miR-339-3p, miR-339-5p, miR-519a, miR-1238, miR-99a#, miR-134, miR-604, miR-539, and miR-342. The expression of miR-339-3p was significantly higher in patients with NSCLC than in those with OLs (P < 0.001) and HPs (P = 0.020). ROC analysis revealed an miR-339-3p expression AUC of 0.616 [95% confidence interval (CI): 0.561–0.702]. The diagnostic prediction was increased (AUC = 0.706, 95% CI: 0.649–0.779) in the model combining miR-339-3p expression and other known risk factors (i.e., age, smoking status, and drinking status). Conclusions: MiR-339-3p was significantly upregulated in patients with NSCLC compared with participants without cancer, suggesting a diagnostic prediction value for high-risk individuals. Therefore, miR-339-3p expression could be a potential blood-based biomarker for NSCLC.

【 授权许可】

Unknown