The genome of the Hi5 germ cell line from Trichoplusia ni, an agricultural pest and novel model for small RNA biology

eLife

Yujing Yang¹  Yu Fu²  Phillip D Zamore³  Gwen Farley⁴  Kaycee A Quarles⁴  Junling Wang⁴  Zhiping Weng⁴  Han Zhang⁴ 

[1] Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United States;Bioinformatics Program, Boston University, Boston, United States;Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, United States;RNA Therapeutics Institute and Howard Hughes Medical Institute, University of Massachusetts Medical School, Worcester, United States;

关键词: piRNAs;    Tricoplusia ni;    Lepidoptera;    insecticide resistance;    siRNAs;    T. ni;   

DOI  :  10.7554/eLife.31628

来源: DOAJ

【 摘 要 】

We report a draft assembly of the genome of Hi5 cells from the lepidopteran insect pest, Trichoplusia ni, assigning 90.6% of bases to one of 28 chromosomes and predicting 14,037 protein-coding genes. Chemoreception and detoxification gene families reveal T. ni-specific gene expansions that may explain its widespread distribution and rapid adaptation to insecticides. Transcriptome and small RNA data from thorax, ovary, testis, and the germline-derived Hi5 cell line show distinct expression profiles for 295 microRNA- and >393 piRNA-producing loci, as well as 39 genes encoding small RNA pathway proteins. Nearly all of the W chromosome is devoted to piRNA production, and T. ni siRNAs are not 2´-O-methylated. To enable use of Hi5 cells as a model system, we have established genome editing and single-cell cloning protocols. The T. ni genome provides insights into pest control and allows Hi5 cells to become a new tool for studying small RNAs ex vivo.

【 授权许可】

Unknown