Re-Emergence of Salmonellosis in Hog Farms: Outbreak and Bacteriological Characterization

Microorganisms

SuzanaSatomi Kuchiishi¹  AdriennyTrindade Reis²  Caroline Reichen³  Mariana Meneguzzi³  RobertoMaurício Carvalho Guedes⁴  Caroline Pissetti⁵  JalusaDeon Kich⁶  Raquel Rebelatto⁶  JoiceAparecida Leão⁷  Julian Trachsel⁸ 

[1] Centro de Diagnóstico de Sanidade Animal, CEDISA, Concórdia 89715-899, SC, Brazil;Centro de Diagnóstico e Monitoramento Animal, CDMA, Belo Horizonte 30411-191, MG, Brazil;Curso de Medicina Veterinária, Instituto Federal Catarinense, Concórdia 89703-720, SC, Brazil;Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil;Departamento de Medicina Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre 91540-000, RS, Brazil;Embrapa Suínos e Aves, Empresa Brasileira de Pesquisa Agropecuária, Concórdia 89715-899, SC, Brazil;Laboratório Integralab, Cascavel 85816-430, PR, Brazil;National Animal Disease Center, Food Safety and Enteric Pathogens, Ames, IA 50010, USA;

关键词: Salmonella;    swine;    PFGE;    MLST;    WGS;   

DOI  :  10.3390/microorganisms9050947

来源: DOAJ

【 摘 要 】

Clinical salmonellosis has been increasing significantly in Brazil in recent years. A total of 130 outbreaks distributed among 10 swine-producing states were investigated. One representative Salmonella isolate from each outbreak was characterized through serotyping, antimicrobial resistance profiles, PFGE, and WGS. From 130 outbreaks: 50 were enteric, 48 were septicemic, 17 cases were characterized as hepato-biliary invasive, 13 as nodal and two were not classified. The most prevalent serovars were a monophasic variant of S. typhimurium (55/130), Choleraesuis (46/130), and Typhimurium (14/130). Most of the strains (86.92%) demonstrated a high rate of multi-drug resistance. The identification of a major Choleraesuis clonal group in several Brazilian states sharing the same resistance genes suggested that these strains were closely related. Six strains from this clonal group were sequenced, revealing the same ST-145 and 11 to 47 different SNPs. The detected plasmid type showed multiple marker genes as RepA_1_pKPC-CAV1321, the first to be reported in Salmonella. All AMR genes detected in the genomes were likely present on plasmids, and their phenotype was related to genotypic resistance genes. These findings reveal that salmonellosis is endemic in the most important pig-producing states in Brazil, emphasizing the need to make data available to aid in reducing its occurrence.

【 授权许可】

Unknown