Antioxidant and anticancer activities of chamomile (Matricaria recutita L.)

BMC Research Notes

Chandraprabha Murali¹  Basma Awad¹  Amr Amin¹  Ameera Al Mansoori¹  Bayan Al-Dabbagh²  Mohamed Elhaw²  Ismail A. Elhaty² 

[1] Department of Biology, College of Science, UAE University;Department of Chemistry, College of Science, UAE University;

关键词: Antioxidants;    Anticancer;    Medicinal plants;    Traditional medicine;   

DOI  :  10.1186/s13104-018-3960-y

来源: DOAJ

【 摘 要 】

Abstract Objectives The present study aimed at determining the antioxidant activity, total phenols and flavonoids and to evaluate the antiproliferative activity of ethanolic extract of Matricaria recutita L. (chamomile). The antioxidant activities were measured using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The total phenolic content was measured by the Folin–Ciocalteu assay. The flavonoid content was determined using the aluminum chloride method. The MTT assay was used to estimate the antiproliferative activities against human hepatoma (HepG2) cancer cell line. We assessed the mode of action of the extract as a cancer preventive agent and reported its ability to regulate tumor angiogenesis by down regulating in a dose dependent manner the expression of some proteins involved in the process. Results The percentage inhibition of DPPH scavenging activity was dose-dependent ranging between (94.8% ± 0.03) at 1.50 mg/mL and (84.2% ± 0.86) at 0.15 mg/mL. It showed high polyphenols (21.4 ± 0.327 mg GAE/g) and high flavonoids content (157.9 ± 2.22 mg QE/g). Effect of extract was investigated against HepG2 cells. A dose-dependent reduction in cell viability was recorded in cells treated with the extract. The IC50 was ~ 300 µg/mL. It significantly inhibited the level of important prerequisite angiogenesis markers both in HepG2 cells and ex vivo.

【 授权许可】

Unknown