Rescue of Cajanus cajan (L) Millspaugh  Cajanus platycarpus (Benth.) van der Maesen Hybrid through Embryo Culture

Taiwania

P. Balarama Swamy Yadav¹  V. Padmaja¹ 

[1] Department of Botany, Andhra University, Visakhapatnam -530 003, India.;

关键词: Cajanus cajan;    Cajanus platycarpus;    Immature embryos;    Embryo rescue;   

DOI  :  10.6165/tai.2002.47(3).219

来源: DOAJ

【 摘 要 】

Genetic improvement of Cajanus cajan (L.) Millspaugh through gene introgression involving wild species of the genus using conventional methods is hampered due to the prevailing post-zygotic barriers. An in vitro technique has been used for rescuing the hybrid embryos between C. cajan and C. platycarpus, that are otherwise destined to abort under in vivo conditions. 11 to 15 days old embryos developed into only undifferentiated callus on both MS and B5 medium supplemented with 2,4-D/IAA alone and in combination with Kinetin. On the other hand, 16-20 days old embryos could be successfully rescued on MS/B5 + 1.0 mgL-1 IAA + 0.5 or 1.0 mgL-1 Kinetin. Successful embryo rescue operation is largely governed by age of the explant, apart from other decisive factors like hormonal combinations and their concentrations in the culture medium.

【 授权许可】

Unknown