USING SUCROSE FOR THE GLUCOSE TOLERANCE TEST DETERMINATION IN BIOCHEMISTRY CLASSES

Journal of Biochemistry Education

C.C. Domingues¹  P.S.C. Preté¹  D.V. Macedo¹  A.C.S. Souza¹  A.A. Alves¹  F.L. Lazarim¹  P.G. Gandra¹  R. Hohl¹  E. de Paula¹  L.S. Tessuti¹  C.M.S. Cereda¹  D.R. Araújo¹  M. Stancanelli¹  F.O.C. Silva¹ 

[1] Departamento de Bioqumica, Instituto de Biologia / Unicamp, CP 6109 13083-970, Campinas, SP;

关键词: GLUCOSE TOLERANCE, BIOCHEMISTRY CLASSES;   

DOI  :  10.16923/reb.v2i2.137

来源: DOAJ

【 摘 要 】

The development of enzymatic, portable, non-expensive kits for glucose dosage allowed this test to beconducted outside the laboratory. Besides, the increasing number of diabetic patients making use ofthese kits turn their prices quite aordable, nowadays. In a previous work we have reported the use ofglucose kits inside the classroom, for the Glucose Tolerance Tests determination, in association withaerobic exercises, as a starting point to teach metabolism (Alves, A.A. et al., Annals of the XXIXSBBq Meeting, 2000). That experience has been successfully employed in the last ve years withstudents from careers such as Biology, Physical Education, Nursing and Medicine, at Unicamp. Wenow extend those observations to the use of sucrose, instead of glucose, as the sugar given in overchargeat the beginning of the experiment (1.15 and 2.3 g/kg weight for glucose and sucrose, respectively).Portable reectance meters provided accurate enzymatic measurement of glucose with a drop of blood.Since the enzyme (glucose oxydase) is specic for glucose (and not for fructose, for instance), the plotsobtained after sucrose intake are very similar to those with glucose. The advantages in using sucroseare: it is cheaper than glucose and suitable for the use outside the lab (easy to nd) and it does notinduce the characteristic unsettled stomach/nausea caused by glucose. Besides, the use of sucrosedoes not invalidate the classical use of the test, since sucrose is cleaved by invertase (giving rise toglucose and fructose) in the duodenum, where glucose units can be absorbed, giving rise the bloodprole evaluated in the tolerance test.

【 授权许可】

Unknown