Performance of the ELISA test for the diagnosis of cysticercosis using experimentally and naturally cattle infected with metacestode of Taenia saginata

Semina: Ciências Agrárias

Anselmo Vasconcelos Rivetti Júnior¹  Luís Augusto Nero²  Tatiane de Oliveira Santos²  Rafaella Paola Meneguete dos Guimarães-Peixoto²  Letícia Ferreira da Silva²  Paulo Sérgio de Arruda Pinto²  Emílio Campos Acevedo-Nieto² 

[1] Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário;Universidade Federal de Viçosa;

关键词: Taenia saginata;    ELISA;    Bovine cysticercosis;    Cut-off;   

DOI  :  10.5433/1679-0359.2015v36n2p807

来源: DOAJ

【 摘 要 】

The bovine taeniasis-cysticercosis complex can be defined as a set of pathological changes caused by the adult form of Taenia saginata in humans and their larval form in animals (Cysticercus bovis). Data on the occurrence of bovine cysticercosis comes from the records of veterinary inspection of meat in slaughterhouses under fiscalization, where some positive cases may go unnoticed, especially in moderate infections. So, it is relevant the use of serological tests that have greater sensitivity than the post-mortem routine exams. Studies have shown the possible application of the ELISA test as a tool for epidemiological studies of the parasitosis and in the identification of animals with cysts. The aim of this study is to determine the detection threshold of the indirect ELISA test, using experimentally and naturally infected animals in detecting cases of cysticercosis. The sensitivity of the test to naturally infected animals using a cut-off 1 and 2 added with 2 standart-deviation (SD) was of 12% and 24.4%, respectively. However, when using the cut-off of 1 and 2 added with 3 SD, the test sensitivity dropped, represented by 14.4% and 1.99% of the test sensitivity. As for the samples from experimentally infected animals, using the cut-off 1 and 2 added with 2 SD the sensitivity was 55.9% and 92.5%; and adding 3 SD the values found were 31.2% and 86%, respectively. The specificity of the test in all situations tested was 100%. It is important to take into account the right choice of control sera used in the ELISA test, whereas, according to its application, it is necessary to increase the sensitivity or specificity.

【 授权许可】

Unknown