Association between Mycobacterium tuberculosis genotype and diabetes mellitus/hypertension: a molecular study

BMC Infectious Diseases

Virasakdi Chongsuvivatwong¹  Edward McNeil¹  Prasit Palittapongarnpim²  Angkana Chaiprasert³  Weizheng Ou⁴  Wan Qin⁴  Huijuan Chen⁵  Jinlan Li⁵  Shiguang Lei⁵  Shengqiong Guo⁵  Rujuan Luo⁶  Siyu Zhang⁶  Komwit Surachat⁷ 

[1] Department of Epidemiology, Faculty of Medicine, Prince of Songkla University;Department of Microbiology, Faculty of Science, Mahidol University;Department of Research and Development Affairs, Faculty of Medicine, Siriraj Hospital, Mahidol University;Guiyang Public Health Clinical Center;Guizhou Provincial Center for Disease Control and Prevention;Liupanshui Center for Disease Control and Prevention;Molecular Evolution and Computational Biology Research Unit, Faculty of Science, Prince of Songkla University;

关键词: Association;    Genotype;    Lineage;    Drug resistance;    Tuberculosis;    NCD;   

DOI  :  10.1186/s12879-022-07344-z

来源: DOAJ

【 摘 要 】

Abstract Background A paucity of studies focused on the genetic association that tuberculosis (TB) patients with non-communicable diseases (NCDs) are more likely to be infected with Mycobacterium tuberculosis (MTB) with more potent virulence on anti-TB drug resistance than those without NCDs. The study aimed to document the predominant genotype, determine the association between MTB genotypes and NCD status and drug resistance. Methods We conducted a molecular study in 105 TB patients based on a cross-sectional study focused on the comorbid relationship between chronic conditions and TB among 1773 subjects from September 1, 2019 to August 30, 2020 in Guizhou, China. The participants were investigated through face-to-face interviews, followed by NCDs screening. The DNA of MTB isolates was extracted prior to genotyping using 24 loci MIRU-VNTR. The subsequent evaluations were performed by phylogenetic trees, combined with tests of statistical power, Chi-square or Fisher and multivariate logistic regression analysis. Results The Beijing family of Lineage 2 (East Asia) was the predominant genotype accounting for 43.8% (46/105), followed by Lineage 4 (Euro-America) strains, including Uganda I (34.3%, 36/105), and the NEW-1 (9.5%, 10/105). The proportion of Beijing strain in patients with and without NCDS was 28.6% (8/28) and 49.4% (38/77), respectively, with a statistical power test value of 24.3%. No significant association was detected between MTB genotype and NCD status. A low clustering rate (2.9%) was identified, consisting of two clusters. The rates of global, mono-, poly- and multi-drug resistance were 16.2% (17/105), 14.3% (15/105), 1.0% (1/105) and 4.8% (5/105), respectively. The drug-resistant rates of rifampicin, isoniazid, and streptomycin, were 6.7% (7/105), 11.4% (12/105) and 5.7% (6/105), respectively. Isoniazid resistance was significantly associated with the Beijing genotype of Lineage 2 (19.6% versus 5.1%). Conclusions The Lineage 2 East Asia/Beijing genotype is the dominant genotype of the local MTB with endogenous infection preponderating. Not enough evidence is detected to support the association between the MTB genotype and diabetes/hypertension. Isoniazid resistance is associated with the Lineage 2 East Asia/Beijing strain.

【 授权许可】

Unknown