Effect of Transglutaminase Cross-Linking in Protein Isolates from a Mixture of Two Quinoa Varieties with Chitosan on the Physicochemical Properties of Edible Films

Coatings

JuanVicente Méndez-Méndez¹  Georgina Calderón-Domínguez²  Prospero Di Pierro³  LuisFelipe Delgado-Sánchez⁴  BlancaE. García-Almendárez⁴  Aldo Amaro-Reyes⁴  RaquelAdriana Ríos-Romo⁴  Carlos Regalado-González⁴  Monserrat Escamilla-García⁴ 

[1] Centro de Nanociencias y Micro y Nanotecnologías, Instituto Politécnico Nacional, Av. Luis Enrique Erro s/n, Nueva Industrial Vallejo, Ciudad de Mexico 07738, Mexico;Departamento de Ingeniería Bioquímica, Instituto Politécnico Nacional, Av. Wilfrido Massieu Esq. Cda. Miguel Stampa S/N, Gustavo A. Madero, Ciudad de Mexico 07738, Mexico;Department of Chemical Sciences, University of Naples “Federico II”, 80126 Naples, Italy;Facultad de Química, Departamento de Investigación y Posgrado en Alimentos, Universidad Autónoma de Querétaro, C.U., Cerro de las Campanas S/N, Col. Las Campanas, Querétaro 76010, Mexico;

关键词: edible films;    quinoa protein;    chitosan;    transglutaminase;   

DOI  :  10.3390/coatings9110736

来源: DOAJ

【 摘 要 】

The growing demand for minimally processed foods with a long shelf life and environmentally friendly materials has forced industry to develop new technologies for food preservation and handling. The use of edible films has emerged as an alternative solution to this problem, and mixtures of carbohydrates and proteins, may be formulated to improve their properties. The objective of this work was to evaluate the effect of protein cross-linking with transglutaminase (TG) of two varieties of quinoa protein isolate (Chenopodium quinoa) [Willd (QW), and Pasankalla (QP)] on the physicochemical and barrier properties of edible films based on chitosan (CT)-quinoa protein. The evaluated properties were water vapor permeability (WVP), solubility, adsorption, roughness determined by atomic force microscopy, and the interactions among the main film components determined by Raman spectroscopy. The results indicated that TG interacted with lysine of QW and QP. CT:QW (1:5, w/w) showed the lowest solubility (14.02 ± 2.17% w/w). WVP varied with the composition of the mixture. The WVP of CT:quinoa protein ranged from 2.85 to 9.95 × 10⁻¹¹ g cm Pa⁻¹ cm⁻² s⁻¹ without TG, whereas adding TG reduced this range to 2.42−4.69 × 10⁻¹¹ g cm Pa⁻¹ cm⁻² s⁻¹. The addition of TG to CT:QP (1:10, w/w) reduced the film surface roughness from 8.0 ± 0.5 nm to 4.4 ± 0.3 nm. According to the sorption isotherm, the addition of TG to CT-QW films improved their stability [monolayer (X_m) = 0.13 ± 0.02 %]. Films with a higher amount of cross-linking showed the highest improvement in the evaluated physical properties, but interactions among proteins that were catalyzed by TG depended on the protein source and profile.

【 授权许可】

Unknown