A novel monoclonal antibody against the von Willebrand Factor A2 domain reduces its cleavage by ADAMTS13

Journal of Hematology & Oncology

Lijun Xia¹  Lulu Zhang¹  Changgeng Ruan¹  Zhenni Ma¹  Jian Su¹  Yiming Zhao¹  Fei Shen¹ 

[1] Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University;

关键词: von Willebrand factor;    Monoclonal antibody;    ADAMTS13;   

DOI  :  10.1186/s13045-017-0407-1

来源: DOAJ

【 摘 要 】

Abstract We developed a novel murine monoclonal antibody (mAb) against the C-terminal α-helix of the human von Willebrand factor A2, designated SZ-179. We showed that SZ-179 inhibited the interactions between VWF and ADAMTS13 and prevented the degradation of high molecular weight VWF multimers. Importantly, SZ-179 reduced the proteolysis of VWF-R1597W mutant by rADAMTS13 dose-dependently under native conditions. Our findings reveal a potential therapeutic target for bleeding disorders.

【 授权许可】

Unknown