Exploring the Behavior of Bovine Serum Albumin in Response to Changes in the Chemical Composition of Responsive Polymers: Experimental and Simulation Studies

Polymers

P. Madhusudhana Reddy¹  Chi-Jung Chang¹  Wan-Chi Wu¹  Awanish Kumar²  Shih-Rong Hsieh³  Hui-Yi Lin⁴ 

[1] Department of Chemical Engineering, Feng Chia University, 100, Wenhwa Road, Seatwen, Taichung 40724, Taiwan;Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;Department of Surgery, Taichung Veterans General Hospital, 1650 Taiwan Boulevard Section 4, Taichung 40705, Taiwan;School of Pharmacy, China Medical University, 91, Hsueh-Shih Road, Taichung 40402, Taiwan;

关键词: protein;    responsive polymer;    thermal stability;    folding;    unfolding;    biomedical applications;   

DOI  :  10.3390/polym8060238

来源: DOAJ

【 摘 要 】

Knowledge of the interactions between polymer and protein is very important to fabricate the potential materials for many bio-related applications. In this regard, the present work investigated the effect of copolymers on the conformation and thermal stability of bovine serum albumin (BSA) with the aid of biophysical techniques such as fluorescence spectroscopy, circular dichroism (CD) spectroscopy and differential scanning calorimetry (DSC). In comparison with that of copolymer PGA-1.5, our fluorescence spectroscopy results reveal that the copolymer PGA-1, which has a lower PEGMA/AA ratio, shows greater influence on the conformation of BSA. Copolymers induced unfolding of the polypeptide chain of BSA, which was confirmed from the loss in the negative ellipticity of CD spectra. DSC results showed that the addition of PGA-1 and PGA-1.5 (0.05% (w/v) decreased the transition temperature by 14.8 and 11.5 °C, respectively). The results from the present study on the behavior of protein in response to changes in the chemical composition of synthetic polymers are significant for various biological applications such as enzyme immobilization, protein separations, sensor development and stimuli-responsive systems.

【 授权许可】

Unknown