IFT trains in different stages of assembly queue at the ciliary base for consecutive release into the cilium

eLife

Yu-Yang Jiang¹  Deborah A Cochran²  George B Witman³  Bing Zhu⁴  Tyler Picariello⁴  Junmin Pan⁴  Jonathon D Walsh⁵  Jenna L Wingfield⁵  Karl Lechtreck⁵  Ilaria Mengoni⁵  Heather Bomberger⁵  Peter Kner⁶  Jason M Brown⁶  Jacek Gaertig⁷  Jonathan Eggenschwiler⁷ 

[1] College of Engineering, University of Georgia, Athens, United States;Department of Biology, Salem State University, Salem, United States;Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China;Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, United States;Department of Cellular Biology, University of Georgia, Athens, United States;Department of Genetics, University of Georgia, Athens, United States;MOE Key Laboratory of Protein Sciences, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China;

关键词: flagella;    IFT;   

DOI  :  10.7554/eLife.26609

来源: DOAJ

【 摘 要 】

Intraflagellar transport (IFT) trains, multimegadalton assemblies of IFT proteins and motors, traffic proteins in cilia. To study how trains assemble, we employed fluorescence protein-tagged IFT proteins in Chlamydomonas reinhardtii. IFT-A and motor proteins are recruited from the cell body to the basal body pool, assembled into trains, move through the cilium, and disperse back into the cell body. In contrast to this ‘open’ system, IFT-B proteins from retrograde trains reenter the pool and a portion is reused directly in anterograde trains indicating a ‘semi-open’ system. Similar IFT systems were also observed in Tetrahymena thermophila and IMCD3 cells. FRAP analysis indicated that IFT proteins and motors of a given train are sequentially recruited to the basal bodies. IFT dynein and tubulin cargoes are loaded briefly before the trains depart. We conclude that the pool contains IFT trains in multiple stages of assembly queuing for successive release into the cilium upon completion.

【 授权许可】

Unknown