Kinetics of Circulating MicroRNAs in Response to Cardiac Stress in Patients With Coronary Artery Disease

Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Jan‐Malte Sinning¹  Lisa Schäfer¹  Christoph Hammerstingl¹  Anna Flender¹  Nikos Werner¹  Felix Jansen¹  Theresa Schmitz¹  Georg Nickenig¹  Robert Schueler¹  Han Wang¹ 

[1] Department of Internal Medicine II, Rheinische Friedrich‐Wilhelms University, Bonn, Germany;

关键词: coronary atherosclerosis;    coronary heart disease risk;    microRNA;   

DOI  :  10.1161/JAHA.116.005270

来源: DOAJ

【 摘 要 】

BackgroundCirculating microRNAs (miRNAs/miRs) are regulated in patients with coronary artery disease. The impact of transient coronary ischemia on circulating miRNA levels is unknown. We aimed to investigate circulating miRNA kinetics in response to cardiac stress in patients with or without significant coronary stenosis. Methods and ResultsEighty of 105 screened patients with stable coronary artery disease underwent dobutamine stress echocardiography before coronary angiography. Nine circulating vascular miRNAs (miRNA‐21, miRNA‐26, miRNA‐27a, miRNA‐92a, miRNA‐126‐3p, miRNA‐133a, miRNA‐222, miRNA‐223, and miRNA‐199‐5p) were quantified in plasma by reverse transcription polymerase chain reaction before, immediately after, and 4 and 24 hours after dobutamine stress echocardiography. Quantitative polymerase chain reaction revealed increased miRNA‐21, miRNA‐126‐3p, and miRNA‐222 levels at 24 hours after dobutamine stress echocardiography in all patients. On coronary angiography, significant coronary artery stenoses (>80% diameter stenosis) were found in 41 patients. Stratifying patients according to the prevalence of significant stenoses, patients with stenosis showed an increase of circulating miRNA‐21, miRNA‐126‐3p, and miRNA‐222 in response to cardiac stress. In patients without significant stenoses (<50% diameter stenosis), miRNA‐92a levels gradually increased in response to cardiac stress. ConclusionsmiRNAs are distinctly released into the circulation in response to cardiac stress depending on the prevalence of significant coronary stenoses.

【 授权许可】

Unknown