Point-of-Need DNA Testing for Detection of Foodborne Pathogenic Bacteria

Sensors

Nikola Knezevic¹  Ioanna Giouroudi¹  Vasa Radonic¹  Ivana Gadjanski¹  Marisa Manzano²  Milica Zivkovic³  Devon Kavanaugh⁴  Priya Vizzini⁴  Nalini Ramarao⁴  Jasmina Vidic⁴ 

[1] BioSense—Research and Development Institute for Information Technologies in Biosystems, University of Novi Sad, 21000 Novi Sad, Serbia;Dipartimento di Scienze AgroAlimentari, Ambientali e Animali, Università di Udine, 33100 Udine, Italy;Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, 11000 Belgrade, Serbia;Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France;

关键词: biosensor;    point-of-need DNA detection;    DNA/RNA extraction;    DNA amplification;    microfluidic;    food security;   

DOI  :  10.3390/s19051100

来源: DOAJ

【 摘 要 】

Foodborne pathogenic bacteria present a crucial food safety issue. Conventional diagnostic methods are time-consuming and can be only performed on previously produced food. The advancing field of point-of-need diagnostic devices integrating molecular methods, biosensors, microfluidics, and nanomaterials offers new avenues for swift, low-cost detection of pathogens with high sensitivity and specificity. These analyses and screening of food items can be performed during all phases of production. This review presents major developments achieved in recent years in point-of-need diagnostics in land-based sector and sheds light on current challenges in achieving wider acceptance of portable devices in the food industry. Particular emphasis is placed on methods for testing nucleic acids, protocols for portable nucleic acid extraction and amplification, as well as on the means for low-cost detection and read-out signal amplification.

【 授权许可】

Unknown