Loading Rates of Dust and Bioburden in Dwellings in an Inland City of Southern Europe

Atmosphere

Célia Alves¹  Estela Vicente¹  Carla Candeias²  Beatriz Almeida³  LilianaAranha Caetano³  Carla Viegas³  Elisabete Carolino³  Marta Dias³ 

[1] Centre for Environmental and Marine Studies (CESAM), Department of Environment and Planning, University of Aveiro, 3810-193 Aveiro, Portugal;Geobiosciences, Geotechnologies and Geoengineering Research Centre (GeoBioTec), Department of Geosciences, University of Aveiro, 3810-193 Aveiro, Portugal;H&TRC—Health & Technology Research Center, ESTeSL—Escola Superior de Tecnologia da Saúde, Instituto Politécnico de Lisboa, 1990-096 Lisbon, Portugal;

关键词: dwellings;    settleable dust;    bioburden;    electrostatic dust collector;    toxigenic fungi;    antifungal resistance;   

DOI  :  10.3390/atmos12030378

来源: DOAJ

【 摘 要 】

Sampling campaigns indoors have shown that occupants exposed to contaminated air generally exhibit diverse health outcomes. This study intends to assess the deposition rates of total settleable dust and bioburden in the indoor air of dwellings onto quartz fiber filters and electrostatic dust collectors (EDCs), respectively. EDC extracts were inoculated onto malt extract agar (MEA) and dichloran glycerol (DG18) agar-based media used for fungal contamination characterization, while tryptic soy agar (TSA) was applied for total bacteria assessment, and violet red bile agar (VRBA) for Gram-negative bacteria. Azole-resistance screening and molecular detection by qPCR was also performed. Dust loading rates ranged from 0.111 to 3.52, averaging 0.675 μg cm⁻² day⁻¹. Bacterial counts ranged from undetectable to 16.3 colony-forming units (CFU) m⁻² day⁻¹ and to 2.95 CFU m⁻² day⁻¹ in TSA and VRBA, respectively. Fungal contamination ranged from 1.97 to 35.4 CFU m⁻² day⁻¹ in MEA, and from undetectable to 48.8 CFU m⁻² day⁻¹ in DG18. Penicillium sp. presented the highest prevalence in MEA media (36.2%) and Cladosporium sp. in DG18 (39.2%). It was possible to observe: (a) settleable dust loadings and fungal contamination higher in dwellings with pets; (b) fungal species considered indicators of harmful fungal contamination; (c) Aspergillus section Candidi identified in supplemented media with voriconazole and posaconazole; (d) specific housing typologies and (e) specific housing characteristics influencing the microbial contamination.

【 授权许可】

Unknown