TIMELESS inhibits breast cancer cell invasion and metastasis by down-regulating the expression of MMP9

Cancer Cell International

Sattout Aman¹  Shujing Li¹  Huijian Wu¹  Bashir Ahmad¹  Liying Mu¹  Bowen Li¹  Yanan Li¹  Yuxi Yang¹  Kangkai Xia¹ 

[1] School of Bioengineering & Key Laboratory of Protein Modification and Disease, Liaoning Province, Dalian University of Technology;

关键词: TIMELESS;    MMP9;    Invasion;    Metastasis;    Breast cancer;   

DOI  :  10.1186/s12935-021-01752-y

来源: DOAJ

【 摘 要 】

Abstract Breast cancer is the first killer leading to female death, and tumor metastasis is one of the important factors leading to the death of patients, but the specific mechanism of breast cancer metastasis is not very clear at present. Our study showed that overexpression of TIMELESS could significantly inhibit the invasion and metastasis of breast cancer cells ZR-75-30 and the assembly of F-actin protein. On the contrary, knockdown of TIMELESS promoted the invasion and metastasis of breast cancer cells. Further study revealed that TIMELESS overexpression decreased the mRNA and protein levels of MMP9. Furthermore, TIMELESS could interact with p65, leading to repress the association of p65 and its acetyltransferase CBP and down-regulating the acetylation level of p65, which inhibited the activation of NF-κB signal pathway. In conclusion, our research showed that TIMELESS may repress the invasion and metastasis of breast cancer cells via inhibiting the acetylation of p65, inhibiting the activation of NF-κB, thus down-regulating the expression of MMP9, and then inhibiting the invasion and metastasis of breast cancer cells.

【 授权许可】

Unknown