Mangifera indica leaves extract and mangiferin modulate CB1 and PPARγ receptors and others markers associated with obesity

Journal of Functional Foods

Rafael Mazioli Barcelos¹  Douglas Costa Gontijo²  Renata Celi Lopes Toledo³  Luciano Gomes Fietto⁴  Lirlândia Pires de Sousa⁵  Larissa Froede Brito⁵  José Humberto de Queiroz⁶  Geraldo Célio Brandão⁶  Alaíde Braga de Oliveira⁷  Sônia Machado Rocha Ribeiro⁷  João Paulo Viana Leite⁸ 

[1] Corresponding author at: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Avenida P.H. Rolfs, s/n – Campus Universitário, CEP 36570-000 Viçosa, MG, Brazil.;Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CEP 31270-901 Belo Horizonte, MG, Brazil;UniBH/Instituto Mineiro de Educação e Cultura, Av. Professor Mário Werneck, 1685, CEP 30455-610 Belo Horizonte, MG, Brazil;Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CEP 31270-901 Belo Horizonte, MG, Brazil;Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Avenida P.H. Rolfs, s/n – Campus Universitário, CEP 36570-000 Viçosa, MG, Brazil;Departamento de Nutrição e Saúde, Universidade Federal de Viçosa, Avenida P.H. Rolfs, s/n – Campus Universitário, CEP 36570-000 Viçosa, MG, Brazil;Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, CEP 31270-901 Belo Horizonte, MG, Brazil;Escola de Farmácia, Universidade Federal de Ouro Preto, Morro do Cruzeiro, s/n, 35400-000 MG, Brazil;

关键词: Mangifera indica;    Mangiferin;    Obesity;    Adipogenesis;    CB1 and PPARγ receptors;    Adiponectin;   

DOI  :  

来源: DOAJ

【 摘 要 】

This study aimed phytochemical characterization (UPLC-DAD-MS/MS) of ethanolic extract of the leaves from Mangifera indica (EMI) and Mangiferin (MAN), analysis of the cytotoxic (MTT) and anti-inflammatory potential (expression of TNF-α) of EMI and MAN in vitro. In addition, was evaluate the effect on the mRNA expression of genes (CB1, PPARγ, adiponectin, resistin and leptin) associated with adipogenesis in adipose tissue of rats fed a cafeteria diet. Thus, wistar rats were treated by gavage with EMI and MAN for several days (according to the post and co-treatments). The adipose tissue was weighed and checked the expression of different markers by RT-PCR. The presence of MAN as major compound in EMI was verified. Both EMI and MAN were not cytotoxic, with lower EMI expression of TNF-α. Furthermore, EMI and MAN had proadipogenic action on post-treatment, while in the co-treatment, EMI attenuated the effect of adipogenesis and MAN increased the adipogenic process.

【 授权许可】

Unknown