Deficiency in the double-stranded RNA binding protein HYPONASTIC LEAVES1 increases sensitivity to the endoplasmic reticulum stress inducer tunicamycin in Arabidopsis

BMC Research Notes

Rikako Hirata¹  Kei-ichiro Mishiba¹  Yuji Iwata¹  Nozomu Koizumi¹ 

[1] Graduate School of Life and Environmental Sciences, Osaka Prefecture University;

关键词: Arabidopsis thaliana;    Endoplasmic reticulum;    HYPONASTIC LEAVES1;    microRNA;    Unfolded protein response;   

DOI  :  10.1186/s13104-019-4623-3

来源: DOAJ

【 摘 要 】

Abstract Objective microRNA (miRNA) is a small non-coding RNA that regulates gene expression by sequence-dependent binding to protein-coding mRNA in eukaryotic cells. In plants, miRNA plays important roles in a plethora of physiological processes, including abiotic and biotic stress responses. The present study was conducted to investigate whether miRNA-mediated regulation is important for the endoplasmic reticulum (ER) stress response in Arabidopsis. Results We found that hyl1 mutant plants are more sensitive to tunicamycin, an inhibitor of N-linked glycosylation that causes ER stress than wild-type plants. Other miRNA-related mutants, se and ago1, exhibited similar sensitivity to the wild-type, indicating that the hypersensitive phenotype is attributable to the loss-of-function of HYL1, rather than deficiency in general miRNA biogenesis and function. However, the transcriptional response of select ER stress-responsive genes in hyl1 mutant plants was indistinguishable from that of wild-type plants, suggesting that the loss-of-function of HYL1 does not affect the ER stress signaling pathways.

【 授权许可】

Unknown