Longitudinal clinico-serological analysis of anti-nucleocapsid and anti-receptor binding domain of spike protein antibodies against SARS-CoV-2

International Journal of Infectious Diseases

Gajanan Sapkal¹  Bipin Tilekar²  Yogesh Gurav²  Kajal Jarande²  Shankar Vidhate²  Kirti Khutwad²  Asha Salunke²  Pragya Yadav²  Ojas Kaduskar²  Chetan Patil²  Snehal Shingade²  Vaishali Bhatt²  Ketki Deshpande²  Priya Abraham²  Gururaj Rao Deshpande²  Varsha Potdar²  Varsha Dange³  Sudeep Kumar⁴  Sadhana Chate⁴  Shilpa Gurav⁴  Sudhir Patsuthe⁵  Pavan Salvi⁶ 

[1] Corresponding author. Dr.Priya Abraham, Director ICMR-National Institute of Virology Pune, Sus Road, Pashan, Pune-411 021, INDIA. Tel: [phone];ICMR-National Institute of Virology, Pune, Maharashtra, Pin 411021, India;Lady Medical officer Health, Pimpri Chinchwad Municipal Corporation, Pune, Maharashtra, India;MIMER Medical College, Talegaon Dabhade, Pune, Maharshatra, India;Naidu Hospital, Pune Municipal Corporation, Pune, Maharashtra, India;Pimpri Chinchwad Municipal Corporation, Pune, Maharashtra, India;

关键词: SARS-CoV-2;    nucleocapsid;    receptor binding domain;    neutralizing antibody response;    IgG antibody response;    240 days;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: Monitoring the antibody responses to SARS-CoV-2 infection and its correlation to clinical spectrum of disease is critical in understanding the disease progression and protection against re-infection. We assessed the nucleocapsid (N) and receptor-binding-domain of spike (SRBD) protein specific IgG and neutralizing antibody (NAb) responses in COVID-19 patients up to 8 months and its correlation with diverse disease spectrum. Methods: During the first wave of the SARS-CoV-2 pandemic, from 284 COVID-19 patients, 608 samples were collected up to 8 months post infection. The patients were categorized as asymptomatic, symptomatic and severe. The N and SRBD IgG and NAb titers were evaluated and correlated with clinical data. Results: A steep increase in antigen specific antibody titers was observed till 40 days post onset of the disease (POD), followed by a partial decline till 240 days. Severe disease was associated with a stronger SRBD IgG response and higher NAb titers. The persistence of antibody response was observed in 76% against N, 80% against SRBD and 80% for NAbs of cases up to 8 months POD. Conclusion: RBD and N protein specific IgG persisted till 240 days POD which correlated with NAb response, irrespective of individual`s symptomatic status indicating overall robust protection against re-infection.

【 授权许可】

Unknown