The Role of Plasma Extracellular Vesicles in Remote Ischemic Conditioning and Exercise-Induced Ischemic Tolerance

International Journal of Molecular Sciences

Eugenio Gutiérrez-Jiménez¹  Peter Mondrup Rasmussen¹  Katrine Tang Stenz¹  Tingting Gu¹  Jesper Just¹  Kim Ryun Drasbek¹  Nina Kerting Iversen¹  Frank de Paoli²  Thomas Ravn Lassen³  Hans Erik Bøtker³  Rikke Bæk⁴  Malene Møller Jørgensen⁴  Mathias Jørgensen⁵  Kristian Vissing⁶  Anders Gravholt⁶  Thomas Skjaerlund Groennebaek⁶  Jesper Emil Jakobsgaard⁶  Jon Herskind⁶  Emil Rindom⁶  Jakob Wang⁶  Peter Sieljacks⁶  Jørgen Kjems⁷  Yan Yan⁷  Jens Randel Nyengaard⁸ 

[1] Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 8000 Aarhus, Denmark;Department of Biomedicine–Physiology, Aarhus University, 8000 Aarhus, Denmark;Department of Cardiology, Aarhus University Hospital, 8200 Aarhus, Denmark;Department of Clinical Immunology, Aalborg University Hospital, 9100 Aalborg, Denmark;Department of Engineering, Aarhus University, 8000 Aarhus, Denmark;Exercise Biology, Department of Public Health, Aarhus University, 8000 Aarhus, Denmark;Interdisciplinary Nanoscience Center, Aarhus University, 8000 Aarhus, Denmark;Sino-Danish College (SDC), University of Chinese Academy of Sciences, Beijing 100190, China;

关键词: remote ischemic conditioning;    resistance exercise;    brain ischemia;    stroke;    plasma extracellular vesicles;    human brain vascular endothelial cells;   

DOI  :  10.3390/ijms23063334

来源: DOAJ

【 摘 要 】

Ischemic conditioning and exercise have been suggested for protecting against brain ischemia-reperfusion injury. However, the endogenous protective mechanisms stimulated by these interventions remain unclear. Here, in a comprehensive translational study, we investigated the protective role of extracellular vesicles (EVs) released after remote ischemic conditioning (RIC), blood flow restricted resistance exercise (BFRRE), or high-load resistance exercise (HLRE). Blood samples were collected from human participants before and at serial time points after intervention. RIC and BFRRE plasma EVs released early after stimulation improved viability of endothelial cells subjected to oxygen-glucose deprivation. Furthermore, post-RIC EVs accumulated in the ischemic area of a stroke mouse model, and a mean decrease in infarct volume was observed for post-RIC EVs, although not reaching statistical significance. Thus, circulating EVs induced by RIC and BFRRE can mediate protection, but the in vivo and translational effects of conditioned EVs require further experimental verification.

【 授权许可】

Unknown