Single Mathematical Parameter for Evaluation of the Microorganisms’ Growth as the Objective Function in the Optimization by the DOE Techniques

Microorganisms

Adrian Augustyniak¹  Maciej Konopacki¹  Rafał Rakoczy¹  Marian Kordas¹  Bartłomiej Grygorcewicz²  Barbara Dołęgowska² 

[1] Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, 71-065 Szczecin, Poland;Department of Laboratory Medicine, Chair of Microbiology, Immunology and Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstańców Wielkopolskich Avenue 72, 70-111 Szczecin, Poland;

关键词: mathematical description;    bacteria cultivation;    growth kinetics;    optimization process;    bacteriology;    bacterial physiology;   

DOI  :  10.3390/microorganisms8111706

来源: DOAJ

【 摘 要 】

The cultivation of bacteria sets a ground for studying biological processes in many scientific disciplines. The development of the bacterial population is commonly described with three factors that can be used to evaluate culture conditions. However, selecting only one of them for the optimization protocol is rather problematic and may lead to unintended errors. Therefore, we proposed a novel mathematical approach to obtain a single factor that could be used as the objective function to evaluate the whole growth dynamic and support the optimization of the biomass production process. The sigmoidal-shape curve, which is the commonly used function to plot the amount of biomass versus time, was the base for the mathematical analysis. The key process parameters, such as maximal specific growth rate and lag-phase duration were established with the use of mathematical coefficients of the model curve and combined to create the single growth parameter. Moreover, this parameter was used for the exemplary optimization of the cultivation conditions of Klebsiella pneumoniae that was cultured to be further used in the production of lytic bacteriophages. The proposed growth parameter was successfully validated and used to calculate the optimal process temperature of the selected bacterial strain. The obtained results indicated that the proposed mathematical approach could be effortlessly adapted for a precise evaluation of growth curves.

【 授权许可】

Unknown