Optimizing Sample Size for Population Genomic Study in a Global Invasive Lady Beetle, Harmonia Axyridis

Insects

Hongran Li¹  Baoping Li¹  Ling Meng¹  Xuguo Zhou²  JohnJ. Obrycki²  Dong Chu³  Wanmei Qu³ 

[1] College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;Department of Entomology, University of Kentucky, Lexington, KY 40546, USA;Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China;

关键词: sample size;    Harmonia axyridis;    2b-RAD;    empirical investigation;    population genomics;    genetic diversity;   

DOI  :  10.3390/insects11050290

来源: DOAJ

【 摘 要 】

Finding optimal sample sizes is critical for the accurate estimation of genetic diversity of large invasive populations. Based on previous studies, we hypothesized that a minimal sample size of 3–8 individuals is sufficient to dissect the population architecture of the harlequin lady beetle, Harmonia axyridis, a biological control agent and an invasive alien species. Here, equipped with a type IIB endonuclease restriction site-associated (2b-RAD) DNA sequencing approach, we identified 13,766 and 13,929 single nucleotide polymorphisms (SNPs), respectively, among native and invasive H. axyridis populations. With this information we simulated populations using a randomly selected 3000 SNPs and a subset of individuals. From this simulation we finally determined that six individuals is the minimum sample size required for the accurate estimation of intra- and inter-population genetic diversity within and across H. axyridis populations. Our findings provide an empirical advantage for population genomic studies of H. axyridis in particular and suggest useful tactics for similar studies on multicellular organisms in general.

【 授权许可】

Unknown