Syndecan-1 Facilitates the Human Mesenchymal Stem Cell Osteo-Adipogenic Balance

International Journal of Molecular Sciences

Chieh Yu¹  LarisaM. Haupt¹  SonH. Pham¹  LynR. Griffiths¹  IanW. Peall¹  RachelK. Okolicsanyi¹ 

[1] Queensland University of Technology, Genomics Research Centre, School of Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia;

关键词: mesenchymal stem cells;    heparan sulfate proteoglycans;    syndecan-1;    osteogenesis;    adipogenesis;    lineage fate;   

DOI  :  10.3390/ijms21113884

来源: DOAJ

【 摘 要 】

Bone marrow-derived human mesenchymal stems cells (hMSCs) are precursors to adipocyte and osteoblast lineage cells. Dysregulation of the osteo-adipogenic balance has been implicated in pathological conditions involving bone loss. Heparan sulfate proteoglycans (HSPGs) such as cell membrane-bound syndecans (SDCs) and glypicans (GPCs) mediate hMSC lineage differentiation and with syndecan-1 (SDC-1) reported in both adipogenesis and osteogenesis, these macromolecules are potential regulators of the osteo-adipogenic balance. Here, we disrupted the HSPG profile in primary hMSC cultures via temporal knockdown (KD) of SDC-1 using RNA interference (RNAi) in undifferentiated, osteogenic and adipogenic differentiated hMSCs. SDC-1 KD cultures were examined for osteogenic and adipogenic lineage markers along with changes in HSPG profile and common signalling pathways implicated in hMSC lineage fate. Undifferentiated hMSC SDC-1 KD cultures exhibited a pro-adipogenic phenotype with subsequent osteogenic differentiation demonstrating enhanced maturation of osteoblasts. In cultures where SDC-1 KD was performed following initiation of differentiation, increased adipogenic gene and protein marker expression along with increased Oil Red O staining identified enhanced adipogenesis, with impaired osteogenesis also observed in these cultures. These findings implicate SDC-1 as a facilitator of the hMSC osteo-adipogenic balance during early induction of lineage differentiation.

【 授权许可】

Unknown