Assessment of Fermented Soybean Meal on Salmonella typhimurium Infection in Neonatal Turkey Poults

Animals

Benjamin Fuente-Martinez¹  Arantxa Morales-Mena¹  Xochitl Hernandez-Velasco²  Roberto Señas-Cuesta³  Guillermo Tellez-Isaias³  KyleD. Teague³  Howard Lester³  BillyM. Hargis³  LucasE. Graham³  ChristineN. Vuong³  Daniel Hernandez-Patlan⁴  Bruno Solis-Cruz⁴  Sergio Martínez-González⁵ 

[1] Centro de Ensenanza, Investigacion y Extension en Produccion Avicola, Facultad de Medicina Veterinaria y Zootecnia (FMVZ), Universidad Nacional Autonoma de Mexico, Ciudad de Mexico 13209, Mexico;Departamento de Medicina y Zootecnia de Aves, FMVZ, Universidad Nacional Autonoma de Mexico, Ciudad de Mexico 04510, Mexico;Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA;Laboratorio 5: LEDEFAR, Unidad de Investigacion Multidisciplinaria, Facultad de Estudios Superiores (FES) Cuautitlan, Universidad Nacional Autonoma de Mexico (UNAM), Cuautitlan Izcalli 54714, Mexico;Unidad Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nayarit, Compostela, Nayarit 63700, Mexico;

关键词: intestinal permeability;    fermented soybean meal;    probiotic;    turkey poults;    Salmonella typhimurium;   

DOI  :  10.3390/ani10101849

来源: DOAJ

【 摘 要 】

This study’s objective was to evaluate the effect of the fermented soybean meal (FSBM) on Salmonella typhimurium (ST) to turkey poults using two models of infection. In the prophylactic model, one-day-old turkeys were randomly allocated to one of four different groups (n = 30 turkeys/group): (1) Control group, (2) FSBM group, (3) Control group challenged with ST (Control + ST), and (4) FSBM group challenged with ST (FSBM + ST). On day 9 of age, all poults were orally challenged with 10⁶ colony forming units (CFU) ST and 24 h post-inoculation, intestinal samples were collected to determine ST recovery and morphometric analysis. Blood samples were collected to evaluate serum fluorescein isothiocyanate-dextran (FITC-d). In the therapeutic model, a similar experimental design was used, but turkeys were orally gavaged 10⁴ CFU ST on day 1, and samples were collected at day 7. FSBM improved performance and reduced leaky gut in both experimental infective models. In the prophylactic model, FSBB induced morphology changes in the mucosa. Although the strains (Lactobacillus salivarius and Bacillus licheniformis) used for the fermentation process showed in vitro activity against ST, no significant effect was observed in vivo. The fermentation with different beneficial bacteria and different inclusion rates of FSBM requires further investigation.

【 授权许可】

Unknown