Antimicrobial, anti-melanogenesis and anti-tyrosinase potential of myco-synthesized silver nanoparticles on human skin melanoma SK-MEL-3 cells

Journal of King Saud University: Science

Mansour K. Gatasheh¹  Sasikala Chinnapan¹  Fatimah S Al-Khattaf²  S. Himalini³  M. Razia³  V. Uma Maheshwari Nallal³  S. Kanimozhi⁴  Venkatalakshmi Ranganathan⁵  Murugesan Chandrasekaran⁶  Ashraf Atef Hatamleh⁷ 

[1] Corresponding authors.;Department of Biochemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;Department of Biotechnology, Mother Teresa Women’s University, Kodaikanal 624101, Tamilnadu, India;Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;Department of Food Science and Biotechnology, Sejong University, Gwangjin-gu, Seoul 05006, Republic of Korea;Department of Pharmaceutical Biology, Faculty of Pharmaceutical Science, UCSI University Kuala Lumpur, Kuala Lumpur, Malaysia;Faculty of Pharmaceutical Sciences, UCSI University, 56000 Cheras, Kulala Lumpur, Malaysia;

关键词: Mycosynthesis;    AgNPs;    Resistance;    Kojic acid;    Anti-melanogenic;    SK-MEL-3 cells;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: Mycosynthesis offers a benevolent alternative for the synthesis of Silver Nanoparticles (AgNPs) when compared to the conventional physical and chemical methods. Fungi secrete enormous amounts of proteins and enzymes that act as reducing and capping agents in AgNPs synthesis. Methods: AgNPs were synthesized using extracellular fungal extract of Fusarium incarnatum. The reaction mixture was optimized using different parameters to obtain better yield. Anti-melanogenic activity of fungal extract, AgNPs and standard drug (Kojic acid) was evaluated against Human skin melanoma SK-MEL-3 cells. AgNPs were synthesized at 30 °C, pH 5 and 1 mM AgNO3 concentration. Then optimization and the characterization were done by UV–Vis, FTIR, XRD, Zeta potential and H-TEM techniques. Results: The average size of AgNPs was 10 nm and predominantly spherical in shape. Myco-synthesized AgNPs showed significant anti-melanogenic activity against human skin melanoma SK-MEL-3 cells with an IC50 value of 17.70 μg/ml. The concentration of AgNPs required to inhibit SK-MEL-3 cells was lower than the concentrations of F. incarnatum extract and standard Kojic acid. Conclusion: Spherical AgNPs with and average size of 4 nm were synthesized using fungal extract of F. incarnatum. Mycosynthesized AgNPs can be considered as effective anti-melanogenic agents owing to their impressive activity against skin melanoma.

【 授权许可】

Unknown