Roles of C/EBP class bZip proteins in the growth and cell competition of Rp (‘Minute’) mutants in Drosophila

eLife

Jorge Blanco¹  Nicholas E Baker¹  Jacob C Cooper² 

[1] Department of Genetics, Albert Einstein College of Medicine, New York, United States;School of Biological Sciences, University of Utah, Salt Lake City, United States;

关键词: cell competition;    ribosomal protein;    xrp1;    c/ebp;    bZip domain;    growth control;   

DOI  :  10.7554/eLife.50535

来源: DOAJ

【 摘 要 】

Reduced copy number of ribosomal protein (Rp) genes adversely affects both flies and mammals. Xrp1 encodes a reportedly Drosophila-specific AT-hook, bZIP protein responsible for many of the effects including the elimination of Rp mutant cells by competition with wild type cells. Irbp18, an evolutionarily conserved bZIP gene, heterodimerizes with Xrp1 and with another bZip protein, dATF4. We show that Irbp18 is required for the effects of Xrp1, whereas dATF4 does not share the same phenotype, indicating that Xrp1/Irbp18 is the complex active in Rp mutant cells, independently of other complexes that share Irbp18. Xrp1 and Irbp18 transcripts and proteins are upregulated in Rp mutant cells by auto-regulatory expression that depends on the Xrp1 DNA binding domains and is necessary for cell competition. We show that Xrp1 is conserved beyond Drosophila, although under positive selection for rapid evolution, and that at least one human bZip protein can similarly affect Drosophila development.

【 授权许可】

Unknown